Device built-in

Product Introduction of Cytena Single-Cell Cloning and Printing System

The Cytena Single-Cell Cloning and Printing System is an innovative automated platform designed for biopharmaceuticals, cell therapy, and basic research. It enables efficient, precise isolation, screening, and printing of single cells while ensuring traceable monoclonality to comply with regulatory requirements (e.g., FDA/EMA).

Core Technological Advantages

1. Non-Contact Piezoelectric Printing Technology
   Utilizing gentle piezoelectric dispensing, the system accurately deposits single cells through a microporous membrane, avoiding mechanical stress from traditional methods like FACS or limiting dilution. Cell viability exceeds 95%, making it ideal for sensitive cells (e.g., stem cells, primary cells).

2. AI-Powered Single-Cell Identification
   Equipped with high-resolution brightfield/fluorescence imaging and AI-based analysis, the system automatically identifies target single cells based on morphology, size, and fluorescence while excluding cell clusters or debris, ensuring monoclonality.

3. Full Automation & Traceability
   The entire workflow—from single-cell isolation to printing into 96/384-well plates—is automated, minimizing human error. Each well is documented with high-resolution images and metadata, supporting CLD (Clonality Documentation) reports for GMP/GLP compliance.

4. Broad Application Compatibility
   Compatible with diverse samples (mammalian cells, yeast, primary cells) and culture conditions (serum-free, 3D microspheres), the system works with standard microplates, slides, or custom substrates, facilitating antibody discovery, CRISPR editing, and cell line development.

Applications

• Cell Line Development: Accelerates stable clone screening, shortening monoclonality verification.
• CAR-T/Regenerative Medicine: Ensures therapeutic cell monoclonality for safer manufacturing.
• Antibody Discovery: High-throughput screening of high-producing single B cells for lead optimization.

User Benefits

Cytena’s system, built on "precision, compliance, and efficiency," reduces weeks-long monoclonality validation to days, cutting R&D costs and serving as an ideal tool for standardized, scalable workflows in modern biolabs.

(Note: Specifications are subject to official technical documentation.)