Device built-in

Introduction to the OptiGen PCR Amplification Genie HT with 12x8 Well Isothermal Module

The OptiGene PCR Amplification Genie HT, combined with its 12x8 well isothermal module, is an instrument focused on rapid and efficient nucleic acid detection, making it particularly suitable for high-throughput application scenarios. Below is a detailed introduction covering its core features, working principles, and application areas.

🔬 Core Features and Technical Advantages

The design of the Genie HT fully considers the needs of modern molecular biology laboratories for efficiency, throughput, and convenience. Its core advantages are mainly reflected in the following aspects:

• High Throughput and Automation Compatibility: The instrument's 12x8 well design means it can process up to 96 samplessimultaneously, making it highly suitable for scenarios requiring large-scale sample testing, such as epidemiological screening or large-scale genotyping. Furthermore, its reagents are supplied in 8-tube strip or 96-well plate formatsand feature low viscosity. This makes them highly compatible with automated liquid handling systems and multi-channel pipettes, significantly improving workflow efficiency and reducing manual operation errors.

• Rapid Isothermal Amplification Technology: Unlike traditional PCR, which requires cycling between three temperatures (denaturation, annealing, extension), the Genie HT is based on isothermal amplification technology. This technology requires only a single, constant temperaturefor the reaction, which can be completed very quickly, often in as little as 15 minutes, drastically reducing detection time.

• Excellent Detection Sensitivity and Specificity: The system utilizes fluorescence detection technology, enabling real-time monitoring of the nucleic acid amplification process. Coupled with melting curve analysisfunctionality, it allows for specific identification and verification of the amplification products after the reaction, eliminating the need for subsequent processing like gel electrophoresis. This reduces contamination risks and improves the accuracy and reliability of the results.

• Portability and Ruggedness: Although the Genie HT is a high-throughput model, its portable counterparts in the series are known for being handheld, compact, and small. They support wireless data transfer modes like WiFi and Bluetooth and can even withstand harsh outdoor climatic conditions, demonstrating excellent environmental adaptability. This suggests that the Genie HT series instruments may inherit a rugged and durable design philosophy.

🧬 Brief Overview of Working Principle

The core of the Genie HT is isothermal amplification technology, such as Loop-mediated Isothermal Amplification (LAMP). This technology utilizes a DNA polymerase with strong strand-displacement activityto achieve exponential amplification of specific nucleic acid sequences at a constant temperature, eliminating the need for a thermal cycler. This method is not only fast but also helps avoid aerosol contamination, making operation simpler and safer.

🧫 Main Application Scenarios

Thanks to its technical features, the Genie HT plays a significant role in various fields:

• Rapid Pathogen Detection: Used for the quick detection of bacterial and viral pathogens in medical diagnostics, food safety monitoring, and environmental microbiology monitoring, enabling early diagnosis and surveillance.

• GMO Detection: Used in the agriculture and food industry for the rapid identification of genetically modified components in plants, animals, and food products.

• Scientific Research: Applicable in basic research for experiments requiring high-throughput nucleic acid amplification, such as gene expression analysis and SNP genotyping.

⚠️ Important Considerations for Use

To achieve optimal performance, the following points should be noted when using the Genie HT:

• Primer Design: The efficiency and specificity of isothermal amplification technologies are highly dependent on well-designed primers. Using specialized primer design software or following established primer design principles is crucial.

• Reaction Optimization: For different detection targets, optimization of reaction conditions (e.g., temperature, time) may be necessary to achieve the best amplification results.

• Contamination Control: Although the closed-tube reaction system reduces the risk of amplicon contamination, adherence to good laboratory practices remains essential.

We hope this introduction provides you with a comprehensive understanding of the OptiGen PCR Amplification Genie HT and its 12x8 well isothermal module. If you have a specific application scenario in mind or would like to know more detailed technical parameters, please feel free to inquire further