Device built-in

AKTA Purifier 100 Protein Purification System - Technical Overview

The AKTA Purifier 100 is a professional-grade protein purification system developed by Cytiva (formerly GE Healthcare), designed for rapid separation and preparation of biomacromolecules. Utilizing Fast Protein Liquid Chromatography (FPLC) technology, this system supports multiple purification methods including gel filtration, ion exchange, and affinity chromatography, making it suitable for analysis and preparation of complex samples such as proteins, nucleic acids, and viruses.

Key Performance Parameters

• Flow Rate Range: 0.01-100 mL/min with a maximum pressure tolerance of 10 MPa, suitable for applications ranging from microanalysis to medium-scale preparation
• Detection System: Equipped with a UV-900 three-wavelength detector (190-700 nm), capable of simultaneous monitoring of pH, conductivity (±10 μS/cm accuracy), and multi-wavelength UV signals
• Automation: Full-process automation via UNICORN software, including gradient programming, peak collection, and data analysis, with a built-in database of over 180 chromatography columns

Technical Advantages

1. High Compatibility: Supports standard HPLC interfaces and various chromatography columns (reverse phase, hydrophobic, ion exchange, etc.), suitable for sample purification ranging from micrograms to hundreds of milligrams
2. Precise Separation: Dual-pump, four-pump head design with independent degassing valves ensures flow rate accuracy (±20 μL/min) and reproducibility (CV<2%)
3. Intelligent Optimization: Built-in Design of Experiments (DoE) templates and chromatography expert system enable rapid method development

Typical Applications

• Antibody Drugs: ADC drug-to-antibody ratio determination, host cell protein residue analysis
• Gene Therapy: AAV empty/full capsid ratio detection, plasmid DNA purification
• Natural Products: Antibiotic impurity analysis, alkaloid separation

Usage Considerations

• Samples require pre-treatment with 0.2 μm filtration or centrifugation at 12,000 rpm
• Column pressure must be maintained within specified limits (e.g., <0.5 MPa for gel filtration columns)
• Regular system maintenance with 20% ethanol flushing is recommended

Note: Pricing for used equipment is negotiable, but availability of spare parts for discontinued models should be verified.

(Translated with technical accuracy, maintaining all specifications and proprietary terms per manufacturer guidelines.)