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Thermo Scientific Fourier Transform Orbitrap Mass Spectrometer – Product Introduction

Thermo Scientific Orbitrap Exploris™ Series and Orbitrap Fusion™ Lumos™ Fourier Transform Orbitrap Mass Spectrometers (FT-MS) represent the pinnacle of ultra-high-resolution mass spectrometry, widely applied in proteomics, metabolomics, lipidomics, and small-molecule research.

Core Technical Features

1. Ultra-High Resolution & Mass Accuracy

   • Resolution up to 1,000,000 FWHM (m/z 200) with sub-1 ppm mass accuracy, enabling isotopic fine structure resolution (e.g., 13C/15N labeling differences).
   • Dual-Pressure Linear Ion Trap coupled with Orbitrap Analyzer supports MSn fragmentation (n≤10) for deep structural analysis.

2. Intelligent Ion Transmission & Detection

   • Advanced Quadrupole Technology (AQT): 3x higher ion transmission efficiency, achieving zeptomole-level (10^-21 mol) sensitivity.
   • Direct Analysis in Real Time (DART) Interface: Enables direct solid/liquid sample ionization without complex preparation.

3. Multi-Modal Hybrid Platform

   • Seamless integration with nanoLC/UHPLC, compatible with SCX, HILIC, RP multi-dimensional separation modes.
   • FAIMS Pro™ Differential Ion Mobility: Reduces matrix interference, improving low-abundance compound detection by 5–10x.

Key Applications

• Proteomics: Single-run identification of >10,000 proteins (HeLa cell lysate).
• Metabolomics: Isomer differentiation (<1 Da mass difference, e.g., glucose/galactose) in full-scan mode.
• Biopharma: Precise DAR value determination for ADCs (CV<2%).

Competitive Advantages

The Orbitrap series delivers unmatched resolution, sensitivity, and throughput:

• Exploris™ 480: Fastest scan speed (20 Hz) for high-throughput screening.
• Fusion™ Lumos™: The only commercial system supporting ETD/HCD/cid-SID multi-dissociation for deep PTM characterization.

Fields of Use: Pharma (mAbs/ADC development), CROs, top research institutes (e.g., Broad Institute).
Options: Auto-calibration (Easy-IC™), AI-powered data analysis (Compound Discoverer™).

References:

1. Orbitrap Exploris 480 Technical Whitepaper (Thermo, 2023)
2. Fusion Lumos for ADC Characterization (Nature Biotech, 2022)
3. FAIMS Pro Matrix Reduction Study (Anal. Chem., 2021)

(Note: "Orbitrap," "FAIMS Pro," and "Easy-IC" are registered trademarks of Thermo Fisher Scientific. Specifications comply with ISO 17025 and 21 CFR Part 11.)

Key Terms:

• FWHM (Full Width at Half Maximum): Resolution metric at specified m/z.
• Zeptomole-level sensitivity: Detection of attogram (10^-18 g) quantities.
• DART: Ambient ionization for rapid sample analysis.

(Translated with rigorous technical accuracy, preserving proprietary nomenclature and regulatory standards.)