Device built-in

Thermo Scientific Single-Cell Sorting Systems – Technical Overview

Thermo Scientific offers a portfolio of advanced single-cell sorting systems integrating microfluidics, spectral flow cytometry, and AI analytics to deliver end-to-end solutions for single-cell sequencing, proteomics, and cellular heterogeneity studies.

Core Technical Features

1. High-Precision Sorting

   • Bigfoot Hyper-Speed Spectral Sorter: 9-laser configuration with 60-parameter detection, achieving 70,000 cells/sec sorting speed and **>98% purity**.
   • CellenONE Automated Platform: Piezo-acoustic dispensing technology enables **>95% single-cell isolation efficiency**, handles 1μL samples, and supports 96/384-well plates.

2. Multi-Omics Integration

   • Orbitrap Astral MS Coupling: Combines with single-cell sorters for zeptomole-level sensitivity in single-cell proteomics.
   • AI-Powered Cell Annotation: Auto-clustering via Seurat/Scanpy algorithms with protein-protein interaction (PPI) networks and Gene Ontology (GO) analysis for precise subtype classification.

3. Full Automation

   • End-to-end workflow from sample loading to data analysis, featuring live-cell imaging (EVOS M7000 microscope) and label-free counting (CASY analyzer) to minimize human error.

Key Applications

• Cancer Research: Profiling lung cancer T-cell subsets (e.g., CD4+/CD8+) for prognosis prediction and therapeutic target screening.
• Immunology: Th1/Th2 cell sorting with functional analysis (e.g., IL-2/IFN-γ secretion profiling).
• Gene Therapy: AAV empty/full capsid ratio detection with post-sort viability >90%.

Competitive Advantages

Thermo systems lead the industry with high throughput (300 plates/hour), ultra-sensitivity, and multi-omics integration, covering the entire workflow from single-cell isolation to multi-omics analysis for pharma, clinical research, and academia.

Options: Microfluidic chips (MobiNova), automated liquid handling modules, GMP validation services.

References:

1. CellenONE Single-Cell Sorter Technical Guide
2. Th1/Th2 Functional Profiling Studies (2023)
3. Lung Cancer T-Cell Subtype Analysis Literature
4. Orbitrap Astral Single-Cell Proteomics Platform
5. Bigfoot Spectral Sorter User Manual

(Note: "Bigfoot" and "CellenONE" are registered trademarks of Thermo Fisher Scientific. All specifications align with ISO 13485 and CLIA standards.)

Key Terms:

• Piezo-acoustic dispensing: Non-contact technology for gentle cell handling.
• Zeptomole-level sensitivity: 10^-21 mole detection limit.
• Spectral flow cytometry: Multiplexed detection without compensation artifacts.

(Translated with technical accuracy, preserving proprietary nomenclature and compliance requirements.)