Device built-in

Thermo Scientific Nanoflow Liquid Chromatography System - Product Introduction

Thermo Scientific offers a range of high-performance nanoflow LC systems, including the UltiMate 3000 RSLCnano and EASY-nLC II, specifically designed for trace sample analysis in proteomics, metabolomics, and related fields.

Core Technical Features

1. Multi-Flow Rate Compatibility

   • UltiMate 3000 RSLCnano: Supports a wide flow rate range (20 nL/min to 50 μL/min), covering nanoflow, capillary, and microflow analysis.
   • EASY-nLC II: Features Intelligent Flow Control (IFC) with gradient precision of 0.1–0.4% RSD, optimized for ultra-low sample volumes (100 nL).

2. High Sensitivity & Resolution

   • ProFlow Technology (UltiMate 3000) enhances nanoflow control, delivering 3x better retention time precision than conventional systems.
   • Splitless design (EASY-nLC II) minimizes sample loss, achieving 10–100x higher sensitivity compared to standard HPLC.

3. Automation & High Throughput

   • 96-well plate-compatible autosampler (UltiMate 3000) with 120-position sample tray and <0.004% carryover.
   • Dual-gradient pump system enables 2D separation for complex matrices (e.g., tissues, biofluids).

4. Smart Integration

   • Chromeleon CDS software provides seamless MS coupling and 21 CFR Part 11-compliant data management.
   • AI-assisted analysis (EASY-nLC II) auto-optimizes methods and generates reports.

Applications

• Proteomics: HeLa cell digest analysis (100 ng sample, >3,000 protein IDs).
• Biopharma: Antibody thermal stability/aggregation profiling.
• Gene Therapy: AAV empty/full capsid ratio detection.

Competitive Advantages

• UltiMate 3000 RSLCnano: The only nano-LC system globally combining ultra-high speed (3x faster) and ultra-high resolution.
• EASY-nLC II: Modular design supports remote maintenance and rapid troubleshooting.

Fields of Use: Pharma/CROs, academic labs.
Options: Autosamplers, multi-channel fluorescence detection, compliance validation.

Note: "RSLCnano" and "EASY-nLC" are registered trademarks of Thermo Fisher Scientific.

References:

1. Thermo Scientific Ultimate 3000 RSLCnano Product Page (2023)
2. Thermo Scientific EASY-nLC II Manual (2024)
3. Orbitrap Fusion Lumos Application Note (2022)

(Translated with technical accuracy, retaining all specifications and proprietary terms per manufacturer guidelines.)