Device built-in

EttaFlow Flow Electroporation System Product Introduction

Core Technological Innovations
The EttaFlow Flow Electroporation System employs continuous-flow electroporation technology, breaking through the throughput limitations of traditional batch electroporators with a processing capacity of 1×10⁷–1×10¹⁰ cells per minute. Its patented flow chamber design combined with adjustable pulse parameters (voltage: 50–3000V, pulse width: 10μs–100ms) achieves 75–95% transfection efficiency for primary cells (e.g., T cells) while maintaining >85% viability, delivering 3x higher throughput than conventional methods.

Key Technical Features

1. Closed Sterile Flow Path:

   • Disposable fluidic cartridges integrate electrodes and sample channels to prevent cross-contamination, meeting GMP-grade cell therapy production requirements
   • Operates at ambient temperatures from -4°C to 40°C, suitable for temperature-sensitive nucleic acid delivery (e.g., mRNA vaccines)

2. Intelligent Parameter Optimization:

   • Preloaded programs for 20 cell types (CHO, HEK293, etc.)
   • Real-time impedance feedback automatically adjusts electric field strength (<2% pulse energy deviation)

3. Multi-Application Adaptability:

   • Expandable configurations for CRISPR RNP, plasmid DNA, and mRNA-specific cartridges
   • Compatible with cell densities ranging from 2×10⁵ to 1×10¹¹ cells/mL

Primary Applications

• Cell Therapy: CAR-T/NK cell engineering (>80% transfection efficiency, >90% viability)
• Vaccine Development: Primary dendritic cell transfection for mRNA-LNP formulations (throughput up to 5×10⁹ cells/hour)
• Industrial Microbiology: Genome editing in E. coli/yeast (40% higher efficiency than cuvette methods)
• Gene Therapy: Suspension HEK293 transfection for AAV vector production

System Configuration

• Main Unit: 21-inch touchscreen with CFR 21 Part 11-compliant data traceability
• Consumables: 1 mL/5 mL/20 mL fluidic cartridges (50–200 transfections per cartridge)
• Expansion Modules: Optional low-temperature (4°C) or high-throughput (96-well adapter) components

ISO 13485 certified, this system has been adopted by companies like Fosun Kite for commercial CAR-T production. The 2025 base model (including 5 cartridges) is priced at approximately ¥1.2–1.5 million RMB. Its compact footprint (45×60 cm) is ideal for Grade B+A cleanroom deployment.

(Note: "EttaFlow" is a registered trademark. Performance data based on primary T-cell validation studies.)

For application-specific protocols or cartridge selection guidance, please contact your regional distributor.