Device built-in

The Thermo Scientific NanoDrop 2000C is a precision analytical instrument widely used in life sciences, biopharmaceuticals, environmental monitoring, and other fields. Known for its microvolume detection capabilities, high-precision measurements, and versatility, it serves as a core tool in modern laboratories. Below is a detailed introduction covering its technical features, applications, and key advantages.

1. Technical Features

1. Microvolume Sample Analysis

   The NanoDrop 2000C requires only 0.5–2 µL of sample for detection. Utilizing patented sample retention technology, it relies on liquid surface tension to hold samples in place on an optical measurement pedestal, eliminating the need for traditional cuvettes or capillary consumables. This design significantly reduces the consumption of precious samples, making it particularly suitable for measuring微量 samples such as DNA, RNA, and proteins.

   In cuvette mode, the instrument supports an adjustable path length of 1–10 mm, enabling the detection of dsDNA concentrations as low as 0.4 ng/µL and covering a broad concentration range (0.4–750 ng/µL). This flexibility makes it suitable for volatile or high-concentration samples.

2. Full Spectrum Detection and Rapid Measurement

   The instrument employs a high-energy xenon flash lamp and a CCD array detector, covering a wavelength range of 190–840 nm (UV-Vis-NIR). It requires no preheating and can be used immediately upon startup. Measurement times are under 5 seconds in pedestal mode and仅 3 seconds in cuvette mode, greatly enhancing experimental efficiency. Its absorbance range reaches 0.02–300 (10 mm equivalent value), with a resolution of <1.8 nm, ensuring data accuracy and reproducibility.

3. Dual-Mode Flexibility

   The NanoDrop 2000C uniquely integrates both pedestal detection and cuvette functionality. The cuvette module supports kinetic studies (e.g., time/temperature curves of enzymatic reactions) and cell culture (OD600 measurements), featuring 37°C constant temperature control and 150–850 rpm stirring capabilities to meet complex experimental requirements.

2. Application Areas

1. Genomics and Molecular Biology

   It rapidly determines the concentration (A260/A280 ratio) and purity (A260/A230 ratio) of DNA and RNA, supporting various nucleic acid types including ssDNA and oligonucleotides. This provides reliable data for downstream experiments such as PCR and sequencing.

2. Proteomics and Drug Development

   It directly measures purified protein concentrations via A280 mode or calculates protein concentrations using dye-based methods (e.g., BCA, Bradford, Lowry) with automatically generated standard curves. This is applicable to drug quality control and protein function studies.

3. Cell Culture and Environmental Monitoring

   The OD600 mode is used for monitoring microbial cell density, while its microvolume detection capability makes it suitable for analyzing low-concentration pollutants in water or soil samples.

3. Key Advantages

• Ease of Use: The intuitive software interface supports custom methods and data export, with a three-step workflow: "dispense–measure–wipe."

• Cost-Effectiveness: It reduces dilution steps and consumable usage, lowering experimental costs. The instrument typically costs between ¥100,000 and ¥150,000.

• Durability and Low Maintenance: The stainless steel pedestal and quartz optical fiber design ensure stability. Daily maintenance仅 requires cleaning with lint-free wipes, making upkeep straightforward.

In summary, the NanoDrop 2000C, with its technological innovations and versatile design, offers an efficient and precise solution for both research and industrial applications, making it an indispensable analytical tool in modern laboratories.