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Shimadzu Nexera LC-40 Ultra High Performance Liquid Chromatograph (UHPLC): A New Benchmark in Intelligent Analysis

Groundbreaking Intelligent UHPLC Platform

Shimadzu's Nexera LC-40 series Ultra High Performance Liquid Chromatograph represents a new height in liquid chromatography technology development, perfectly integrating revolutionary intelligence with exceptional analytical performance. As a leading product in UHPLC technology, this system combines artificial intelligence, Internet of Things, and advanced automation technologies, providing modern analytical laboratories with unprecedented analytical efficiency and operational convenience. The Nexera LC-40 not only inherits Shimadzu's decades of technological accumulation in the chromatography field but also redefines the analytical standards of high-performance liquid chromatography through innovative technology, making it the preferred analytical tool in pharmaceuticals, biotechnology, food safety, environmental monitoring, and other fields.

Breakthrough Technical Performance Parameters

The Nexera LC-40 features outstanding hardware performance, with a maximum system pressure resistance of 130 MPa (approximately 19,000 psi), supporting the use of sub-2μm particle size columns, significantly improving separation efficiency and analysis speed. The flow rate range is wide, from 0.0001 mL/min to 10 mL/min, meeting various application needs from microliter to conventional flow rates. The sampling system has extremely high precision, with a sample carryover rate of less than 0.001%, ensuring the accuracy and reproducibility of analytical results.

The system adopts an innovative fluidic design, with a very small delay volume (<250 μL), which can be further reduced to <100 μL through optional configurations, greatly reducing the need for revalidation during method transfer. The gradient mixing accuracy reaches ±0.1%, ensuring perfect reproducibility of complex gradient methods. The temperature control system accuracy reaches ±0.5°C, providing a solid guarantee for method stability.

Intelligent and Automated Features

The intelligent functionality of the Nexera LC-40 is its most notable highlight. The built-in "Diagnostic Assistant" monitors instrument status in real-time, automatically identifying and warning of potential issues. The "Repair Assistant" function can automatically perform flushing and equilibration operations when abnormalities are detected, significantly reducing manual intervention. The unique "FlowPilot" technology can smoothly change flow rates, effectively protecting chromatographic columns from pressure shocks.

The degree of automation is significantly improved. The intelligent mobile phase management system can monitor solvent levels in real-time and automatically calculate the required volume before analysis to avoid interruptions. The autosampler supports continuous analysis of 162 sample vials or 768 samples (using two 384-well plates), with sampling speed twice as fast as previous generation products, completing a single injection in as little as 5 seconds.

Modular Design and Detector Options

The system adopts a highly modular design, allowing users to flexibly configure according to specific needs:

• SIL-40 series autosampler: Supports online sample preparation functions, including automatic dilution, derivatization, internal standard addition, etc.

• SPD-M40 photodiode array detector: Wavelength range 190-800 nm, acquisition frequency up to 100 Hz, using triple temperature control technology to ensure baseline stability

• RF-40 fluorescence detector: Provides extremely high sensitivity and selectivity, suitable for trace analysis

• RID-40 refractive index detector: Suitable for detection of substances without UV absorption

• CTO-40 column oven: Integrated eCord technology, automatically recording chromatographic column usage history and performance data

Advanced Software Control System

The Nexera LC-40 operates on Shimadzu's LabSolutions Chromatography Data System, fully supporting cGMP/GLP and 21 CFR Part 11 regulatory compliance requirements. The software has a built-in ORACLE® database, providing complete audit trail, electronic signature, and权限管理 functions. Intelligent method development tools can automatically screen the best chromatographic column and mobile phase combinations, greatly shortening method development time.

The application of IoT technology enables remote monitoring, allowing users to view the operating status of multiple instruments in real-time through smart devices, receive alarm information, and optimize the allocation of laboratory resources. Intelligent data processing algorithms can automatically identify peak shape abnormalities, integration problems, and provide optimization suggestions.

Wide Range of Application Fields

The Nexera LC-40 demonstrates excellent performance in multiple fields:

• Pharmaceutical industry: Analysis of active pharmaceutical ingredients, impurity profiling, dissolution testing, bioequivalence studies

• Bioanalysis: Proteomics, metabolomics, biomarker discovery

• Food safety: Pesticide residue detection, additive analysis, nutritional component determination

• Environmental monitoring: Analysis of organic pollutants in water, soil sample testing

• Scientific research: Natural product separation, chemical reaction monitoring, material characterization

Environmental Protection and Energy-Saving Design

The Nexera LC-40 adopts a compact design, with a footprint reduced by approximately one-third compared to previous generation products, effectively saving laboratory space. Energy performance is outstanding, with standby power consumption reduced by more than 68%, conforming to green laboratory concepts. The intelligent sleep function can automatically enter low-power mode when idle, further reducing operating costs.

Technical Summary and Outlook

Shimadzu's Nexera LC-40 Ultra High Performance Liquid Chromatograph creates a comprehensive intelligent analysis platform by deeply integrating cutting-edge hardware performance with artificial intelligence and IoT technology. The system not only provides excellent analytical performance but also sets new standards in automated operation, intelligent diagnostics, and remote monitoring. Its modular design and flexible configuration can meet the diverse needs of laboratories of different scales, providing reliable support from basic quality control to cutting-edge scientific research.

With the continuous development of analytical science, the Nexera LC-40 platform will continue to evolve, incorporating more artificial intelligence algorithms and predictive maintenance functions, further simplifying operational processes, improving analytical efficiency, and providing scientists with more powerful and intelligent analytical tools, promoting analytical chemistry toward higher levels of automation and intelligence.