Device built-in

Thermo Scientific Speedvac DNA 130 Vacuum Concentrator

Introduction

The Thermo Scientific Speedvac DNA 130 Vacuum Concentrator is a specialized instrument designed for modern molecular biology laboratories, particularly for high-throughput genomics applications. It integrates precision temperature control, efficient vacuum technology, and intelligent programming to maximize recovery rates, purity, and efficiency in DNA/RNA sample processing.

1. Core Technology and Working Principle

The system combines vacuum centrifugation, heating, and condensation trapping to achieve gentle, rapid, and efficient sample concentration and drying:

• Vacuum Environment: A high-performance diaphragm vacuum pump reduces pressure within the chamber, significantly lowering the boiling point of solvents. This allows liquids to evaporate quickly even at room temperature, preventing thermal degradation of heat-sensitive DNA molecules.

• Centrifugal Force: A high-speed rotor accommodates various tube formats (e.g., microplates, PCR tubes, centrifuge tubes). Centrifugal force suppresses bumping and cross-contamination while ensuring samples are concentrated uniformly at the bottom of tubes, facilitating easy resuspension and maximizing recovery.

• Heating and Condensation: Controlled radiative heating accelerates evaporation, while an integrated cold trap (as low as -80°C) instantly captures solvent vapors, protecting the vacuum pump and maintaining optimal vacuum levels.

2. Key Features and Advantages

• High-Throughput Capacity: Designed for standard microplates (e.g., 96-well plates) and tube racks, enabling simultaneous processing of up to 126 samples. Ideal for next-generation sequencing (NGS), genotyping, PCR, and other high-volume applications.

• Exceptional Sample Recovery and Integrity: Gentle vacuum centrifugation prevents sample loss, bumping, and cross-contamination. Concentrated DNA forms a uniform pellet for easy resuspension, ensuring high recovery rates and maintaining biological activity.

• Smart and User-Friendly Operation: Features an intuitive color touchscreen interface, allowing users to create, save, and run up to 100 method programs. Real-time monitoring of vacuum, temperature, and time ensures reproducibility and safety with automated shutdown upon completion.

• System Protection and Easy Maintenance: The chemical-resistant vacuum pump and efficient cold trap ensure long-term reliability. Self-diagnostic functions and alarms (e.g., for low vacuum or door issues) enhance operational safety.

3. Typical Applications

• NGS Library Preparation: Concentrating and purifying DNA fragments after enzymatic reactions, ligation, or PCR amplification.

• PCR/RT-PCR Product Purification: Removing primers, dNTPs, and salts while concentrating products for cloning, sequencing, or detection.

• DNA Sequencing Cleanup: Desalting and concentrating cycle sequencing products prior to sequencing.

• Nucleic Acid Labeling and Hybridization: Concentrating labeled cDNA/RNA for microarray experiments.

• General Nucleic Acid Storage: Reducing volumes of dilute samples for long-term storage or transportation.

Conclusion

The Thermo Scientific Speedvac DNA 130 Vacuum Concentrator sets the standard for nucleic acid sample processing. By harmonizing vacuum, centrifugation, and temperature control, it delivers unmatched efficiency, reliability, and sample protection for high-throughput genomics workflows. It is an indispensable tool for ensuring the success of downstream applications such as sequencing, cloning, and PCR, making it a cornerstone of modern molecular biology laboratories.

Note: The translation retains technical accuracy while adapting to English scientific conventions, including standardized terminology (e.g., "vacuum concentrator," "cold trap," "high-throughput") and fluid narrative structure.