Device built-in

Agilent 1260 Infinity II HPLC System (Asset #1033)

The Agilent 1260 Infinity II High-Performance Liquid Chromatography (HPLC) System is a benchmark modular liquid chromatography platform renowned for its exceptional reliability, precision, and flexibility. It serves as an ideal tool for quantitative analysis, method development, and routine testing in various laboratories. Its modular design allows users to customize the system according to specific application needs, ranging from isocratic pumps to quaternary gradient pumps, and from standard autosamplers to high-performance versions.

Core Features and Technological Innovations:

• Ultra-High Precision and Stability: The binary pump employs an active inlet valve and dual-piston serial technology, ensuring minimal flow fluctuations and exceptional flow accuracy. This guarantees outstanding retention time reproducibility and a stable baseline for high-sensitivity detectors (e.g., CAD), forming the foundation for accurate quantitative results.

• Advanced Temperature Control System: The system features a high-performance column compartment capable of precise temperature control (typically from 10°C below ambient to over 80°C). Accurate temperature management is critical for improving peak shape, enhancing separation resolution, and ensuring method reproducibility.

• Intelligent Operation and Data Processing: Controlled by Agilent OpenLab CDS or ChemStation software, the system offers an intuitive user interface, powerful method setup capabilities, real-time monitoring, and comprehensive data processing. Its compliance with 21 CFR Part 11 regulations, including electronic signatures and audit trails, makes it fully suitable for GMP/GLP-compliant pharmaceutical and quality control laboratories.

• Excellent Compatibility and Flexibility: As a fully open platform, the 1260 Infinity II seamlessly integrates with various detectors (including DAD, FLD, RID, MS, and the CAD detector in this package). Its modular design allows for future upgrades or expansions to meet evolving analytical needs.

Applications:

This HPLC system is widely used in pharmaceuticals (content determination, impurity testing), biotechnology (peptide and protein analysis), food safety (additive and pesticide residue testing), environmental monitoring, chemical synthesis, and academic research—virtually any field requiring liquid chromatography separation.

Thermo Scientific Corona Veo RS Charged Aerosol Detector (2022 Model with Peak 1010 Nitrogen Generator)

The Thermo Scientific Corona Veo RS Charged Aerosol Detector (CAD) is a next-generation aerosol-based detector representing a significant leap in technology for analyzing compounds with no or weak UV absorption. Paired with a Peak Scientific 1010 series nitrogen generator, it provides a continuous, pure, and dry nitrogen source without the need for expensive nitrogen cylinders or liquid nitrogen, ensuring low operating costs and exceptional convenience. As a "universal" detector, the CAD responds to virtually all non-volatile or semi-volatile compounds regardless of their optical properties, delivering high sensitivity and consistent responses.

Working Principle:

The CAD operates in three key steps:

1. Nebulization: The eluent from the chromatography column is atomized into fine droplets using nitrogen gas.

2. Evaporation: The droplets pass through a heated drift tube where volatile solvents evaporate, leaving behind dry analyte particles.

3. Detection: The dry particles pass through a detector equipped with an electrometer, where the carried charge is measured and converted into an electrical signal, forming a chromatographic peak. The particle count is proportional to the mass of the analyte, making the response directly reflective of the analyte mass.

Core Advantages and Features:

• Near-Universal Response: Similar to but superior than ELSD (Evaporative Light Scattering Detector), it detects compounds without chromophores, such as sugars, lipids, carbohydrates, surfactants, polymers, and many natural products.

• High Sensitivity: Typically 3-10 times more sensitive than ELSD, enabling detection of lower analyte concentrations with a wider dynamic range.

• Consistent Response Factors: A key advantage over other universal detectors. For compounds of the same mass, the response remains highly consistent regardless of chemical structure. This enables semi-quantitative analysis in the absence of reference standards and simplifies method development.

• Excellent Reproducibility: The 2022 model incorporates the latest technological improvements, ensuring high repeatability of peak areas and retention times for reliable results.

• Perfect Compatibility with Gradient Elution: The CAD is insensitive to solvent gradient changes, providing a stable baseline ideal for complex gradient methods.

• Plug-and-Play Convenience: Compatible with major HPLC systems like Agilent 1260, it connects easily via analog signal interfaces for straightforward installation and operation.

• Cost-Effective Nitrogen Solution: The included Peak 1010 nitrogen generator produces high-purity nitrogen on demand, eliminating the hassle of ordering and managing gas cylinders, ensuring uninterrupted supply, and reducing long-term operational costs.

Applications:

The CAD is ideal for:

• Pharmaceutical Analysis: Quantifying antibiotics, steroids, phospholipids, and excipients (e.g., sugars).

• Lipidomics: Analyzing triglycerides, phospholipids, cholesterol esters, etc.

• Natural Products: Characterizing components in herbal extracts.

• Polymer Analysis: Measuring polymer molecular weight distributions and additives.

• Food and Beverage Testing: Analyzing sugars, sweeteners, amino acids, glycerol, etc.

Combined Advantages

The combination of the Agilent 1260 Infinity II HPLC and the Thermo Scientific Corona Veo RS CAD forms a powerful and complete analytical ecosystem. The 1260 HPLC delivers unparalleled separation reliability, precision, and stability, providing an ideal foundation for the CAD detector. In turn, the CAD expands the analytical capabilities of the 1260 HPLC, enabling it to address challenges unsolvable by traditional UV detectors—achieving high-sensitivity, highly reproducible, and consistent detection of a broader range of compounds.

This 2022 combination offers state-of-the-art performance and advanced technology, making it an exceptional investment for laboratories seeking to enhance their analytical capabilities, particularly in the analysis of non-UV-absorbing compounds.