Device built-in

CTL FluoroSpot Analyzer: Next-Generation Immunoassay System

Key Features

The CTL FluoroSpot Analyzer is an innovative immunoassay instrument that utilizes multiplex fluorescent labeling technology to enable multi-cytokine secretion analysis at single-cell resolution. As an advanced version of ELISPOT technology, this system can simultaneously detect 2-4 different cytokines with sensitivity as high as 1 in 1 million cells, providing multi-dimensional data for immunological research.

Technical Advantages

1. Multiplex Detection: Simultaneous analysis of multiple cytokines (e.g., IFN-γ, IL-2, IL-4) in a single assay
2. High Sensitivity: 10-100 times more sensitive than traditional chromogenic methods
3. Accurate Quantification: Linear correlation between fluorescence intensity and secretion levels
4. High Throughput: Compatible with 6-384 well plates, processing thousands of samples daily
5. High Resolution: 10μm imaging resolution for clear distinction of adjacent spots

Core Specifications

• Detection Channels: Standard 3 fluorescent channels (expandable)
• Dynamic Range: 5-5000 spots/well
• Imaging Speed: <30 seconds/well (96-well plate)
• Light Source Life: LED lifespan >20,000 hours
• Analysis Functions: Automatic counting, size analysis, intensity measurement

Applications

1. Vaccine Development: Evaluating cellular immune responses
2. Cancer Immunology: Detecting tumor-specific T cells
3. Infection Immunity: Studying pathogen-specific responses
4. Autoimmune Diseases: Analyzing autoreactive T cells
5. Transplant Monitoring: Assessing rejection status

Workflow

1. Sample Preparation: Completed in 1-2 days (vs 3-4 days with traditional methods)
2. Detection Analysis: 30-60 minutes per plate
3. Result Interpretation: Multicolor fluorescence visualization of cell subsets

System Components

• Imaging Unit: Integrated fluorescent CCD and temperature control system
• Analysis Software: Automatic counting and statistical analysis
• Reagent Kits: Pre-coated plates, fluorescent antibodies
• Data Management: Compliant with GLP standards

Quality Assurance

The CTL Analyzer is ISO 13485 certified and provides:

• System validation protocols
• Professional technical support
• Regular calibration services
• Method development guidance

With its multiplex detection capability and high sensitivity, the CTL FluoroSpot Analyzer is establishing itself as the next-generation standard tool in immunological research, offering critical technical support for precision medicine and personalized therapies.