Device built-in
CTL FluoroSpot Analyzer: Next-Generation Immunoassay System
Key Features
The CTL FluoroSpot Analyzer is an innovative immunoassay instrument that utilizes multiplex fluorescent labeling technology to enable multi-cytokine secretion analysis at single-cell resolution. As an advanced version of ELISPOT technology, this system can simultaneously detect 2-4 different cytokines with sensitivity as high as 1 in 1 million cells, providing multi-dimensional data for immunological research.
Technical Advantages
- Multiplex Detection: Simultaneous analysis of multiple cytokines (e.g., IFN-γ, IL-2, IL-4) in a single assay
- High Sensitivity: 10-100 times more sensitive than traditional chromogenic methods
- Accurate Quantification: Linear correlation between fluorescence intensity and secretion levels
- High Throughput: Compatible with 6-384 well plates, processing thousands of samples daily
- High Resolution: 10μm imaging resolution for clear distinction of adjacent spots
Core Specifications
- Detection Channels: Standard 3 fluorescent channels (expandable)
- Dynamic Range: 5-5000 spots/well
- Imaging Speed: <30 seconds/well (96-well plate)
- Light Source Life: LED lifespan >20,000 hours
- Analysis Functions: Automatic counting, size analysis, intensity measurement
Applications
- Vaccine Development: Evaluating cellular immune responses
- Cancer Immunology: Detecting tumor-specific T cells
- Infection Immunity: Studying pathogen-specific responses
- Autoimmune Diseases: Analyzing autoreactive T cells
- Transplant Monitoring: Assessing rejection status
Workflow
- Sample Preparation: Completed in 1-2 days (vs 3-4 days with traditional methods)
- Detection Analysis: 30-60 minutes per plate
- Result Interpretation: Multicolor fluorescence visualization of cell subsets
System Components
- Imaging Unit: Integrated fluorescent CCD and temperature control system
- Analysis Software: Automatic counting and statistical analysis
- Reagent Kits: Pre-coated plates, fluorescent antibodies
- Data Management: Compliant with GLP standards
Quality Assurance
The CTL Analyzer is ISO 13485 certified and provides:
- System validation protocols
- Professional technical support
- Regular calibration services
- Method development guidance
With its multiplex detection capability and high sensitivity, the CTL FluoroSpot Analyzer is establishing itself as the next-generation standard tool in immunological research, offering critical technical support for precision medicine and personalized therapies.