Device built-in

Agilent BioTek Cytation5 Multi-Mode Microplate Reader: The Perfect Integration of Cellular Imaging and Microplate Detection

The Agilent BioTek Cytation5 is an innovative multi-mode microplate reader that combines high-performance microplate detection with high-resolution cellular imaging, providing unprecedented experimental solutions for life science research.

Core Technical Features

Cytation5 employs Hybrid Technology™, integrating the high sensitivity of filter-based detection with the flexibility of a monochromator system. Its monochromator bandwidth is continuously adjustable from 9-50nm with 1nm stepping precision, achieving fluorescence detection sensitivity as high as 0.25 pM fluorescein sodium. The instrument features a modular design, allowing users to configure microscope modules, microplate detection modules, or a combination of both according to their needs.

The imaging system is equipped with a 16-bit monochrome CCD camera and interchangeable LED light sources, working in conjunction with Olympus objectives (1.25x-60x) and Semrock filters to support imaging from whole-well observation to cellular details. The 6-position objective turret design enables rapid switching between different magnification levels.

Multi-Mode Detection Capabilities

Cytation5 offers comprehensive detection modes:

• Absorbance detection: Wavelength range 230-999nm, OD resolution 0.0001
• Fluorescence detection: 250-850nm, top detection sensitivity 0.25fmol fluorescein
• Luminescence detection: Can detect as low as 10amol ATP
• Advanced detection: Time-resolved fluorescence, fluorescence polarization, and AlphaScreen/AlphaLISA analysis

The instrument features full automation, including autofocus, auto-exposure, and XYZ automated stage. Dual syringe pump injectors (5-1000μL) support rapid kinetic studies. Temperature control (4°C-65°C) and optional CO₂/O₂ gas control provide ideal conditions for live-cell experiments.

Advanced Imaging Functions

Cytation5 supports multiple imaging modes:

• Fluorescence, brightfield, H&E staining, phase contrast imaging
• Z-stacking capability for 3D spheroid observation
• Image stitching technology for large field-of-view observation

With Gen5 software, it enables automated cell counting, subpopulation analysis, and signal translocation tracking. The innovative Hit-Picking function combines rapid detection with high-resolution imaging, significantly improving experimental efficiency.

Wide Application Areas

Cytation5 is suitable for:

1. Cell biology: Cell proliferation, toxicity testing, apoptosis analysis
2. Drug development: High-throughput screening, drug metabolism studies
3. Molecular detection: Nucleic acid/protein quantification, ELISA
4. Immunology and toxicity testing: Medical device cytotoxicity evaluation
5. Biopharmaceutical R&D: Antibody screening, vaccine development

System Expandability

Cytation5 can integrate with the BioSpa 8 automated incubator for long-term live-cell observation. It is compatible with third-party automation equipment, making it suitable for large-scale screening platforms. Agilent provides comprehensive technical support to ensure optimal instrument performance.

As a next-generation multi-functional cellular imaging and detection system, Cytation5 is driving innovation and advancement in life science research methodologies.