Device built-in

Thermo Scientific Q Exactive Plus High-Resolution Mass Spectrometer Overview

1. Key Features

The Q Exactive Plus is a hybrid quadrupole-Orbitrap high-resolution mass spectrometer developed by Thermo Fisher Scientific, innovatively combining quadrupole ion selection with Orbitrap high-resolution detection. Its core advantages include:

• High Resolution: Resolution up to 140,000 FWHM (upgradeable to 280,000), mass accuracy better than 1 ppm (with internal calibration), capable of resolving isobaric interferences.
• Rapid Scanning: Scan speed of 12 Hz, compatible with UHPLC high-throughput analysis, polarity switching time <1 second (at 35,000 resolution).
• High Sensitivity: Signal-to-noise ratio of 100:1 for 500 fg buspirone in full scan mode, with SIM mode sensitivity enhanced to 30 fg.

2. Technological Innovations

1. Advanced Quadrupole Technology (AQT): Optimizes precursor ion selection and transmission, improving quantification accuracy for low-abundance analytes in complex matrices (e.g., blood, tissues), with adjustable isolation width (0.4 Da to full mass range).
2. Intelligent Ion Optics System:
   • Active Beam Guide (AABG): Reduces noise and extends maintenance intervals.
   • Bent Flatapole: Filters out neutral particles, improving signal-to-noise ratio.
3. Multi-Mode Data Acquisition: Supports data-independent acquisition (DIA), parallel reaction monitoring (PRM), and all-ion fragmentation (AIF), suitable for both targeted and untargeted analyses.

3. Typical Applications

1. Proteomics:
   • Bottom-up proteomics analysis, capable of identifying protein subunits ≤50 kDa with isotopic resolution.
   • Monoclonal antibody characterization, supporting intact protein mode (optional).
2. Pharmaceutical & Clinical:
   • Detection of nitrosamine impurities (e.g., NDMA) in ranitidine, with a detection limit of 0.001 ppb (FDA-compliant).
   • Quantification of catecholamines in plasma, precision <3% RSD.
3. Environmental & Food Safety: Simultaneous analysis of nine nitrosamine residues or pesticide metabolites, with dynamic range covering pg/mL to μg/mL.

4. Service & Support

Thermo Fisher provides a global service network, with core parts delivered within 7 days, and method development support (e.g., semiconductor-grade reagent testing protocols).

Summary

The Q Exactive Plus, with its high resolution, sensitivity, and multi-mode acquisition capabilities, is a benchmark instrument for complex matrix analysis, widely used in research, pharmaceuticals, and environmental monitoring.