Device built-in

GE Axichrom 70/300 Chromatography Column (with Stainless Steel Stand) Product Introduction

1. Product Overview

The GE Axichrom 70/300 Chromatography Column is a high-performance preparative chromatography column from GE Healthcare’s Axichrom series, designed for medium- to large-scale biomolecule purification. Manufactured with premium materials and precision engineering, it ensures high flow rates, excellent resolution, and reproducibility, making it ideal for the separation and purification of proteins, antibodies, vaccines, nucleic acids, and other biomolecules. The included stainless steel stand provides stable support, facilitating easy setup and operation in both laboratory and production environments.

2. Key Features

1. Optimized Column Design

   • Dimensions: 70 mm (inner diameter) × 300 mm (bed height), balancing high loading capacity and resolution, suitable for pilot-scale and small production runs.
   • Material: Constructed from high-purity polymers or glass (model-dependent), offering broad chemical compatibility and resistance to pH 1-14 and common buffers.
   • Sealing System: Dynamic sealing technology prevents leaks, supporting high-pressure operation (up to 0.5 MPa).

2. Stainless Steel Stand Benefits

   • Sturdy Support: Made from 304/316 stainless steel, corrosion-resistant and durable, suitable for lab or GMP environments.
   • Flexible Installation: Adjustable height for compatibility with different systems (e.g., ÄKTA™, BioProcess™ chromatography systems).
   • Mobility: Some models include casters for easy movement within cleanroom areas.

3. Superior Hydrodynamic Performance

   • Uniform Flow Distribution: Optimized flow distributors minimize dead volume, ensuring consistent linear flow rates and improved separation efficiency.
   • Low Backpressure: Supports high flow rates (e.g., up to 300 cm/h in ion exchange chromatography), reducing purification time.

4. Broad Resin Compatibility

   • Compatible with various chromatography media (e.g., GE HiTrap™, Capto™, Sepharose™ series), covering affinity, ion exchange, hydrophobic interaction, and size exclusion chromatography.

3. Applications

• Biopharmaceuticals: Capture and polishing of monoclonal antibodies (mAbs) and recombinant proteins.
• Vaccine Production: Purification of viral vectors and subunit vaccines.
• Gene Therapy: Chromatographic separation of plasmid DNA and mRNA.
• Academic Research: Laboratory-scale protein purification and process development.

4. Operation & Maintenance

1. Column Packing Guidelines

   • Use slurry packing methods, with axial compression technology recommended for uniform bed formation.
   • Adjustable bed height adapters optimize compression ratios, preventing resin collapse.

2. Cleaning & Storage

   • Routine cleaning: 0.1-1 M NaOH or 6 M urea to remove protein residues.
   • Long-term storage: 20% ethanol solution to prevent microbial growth.

3. Accessory Compatibility

   • Standard quick-connect fittings compatible with GE ÄKTA systems and third-party tubing.
   • Optional column guards available for light-sensitive samples.

5. Technical Specifications (Example)

6. Quality & Certifications

• Complies with USP Class VI biocompatibility standards.
• Select models include FDA DMF documentation for GMP compliance.

7. Purchasing Recommendations

• Process Development: Pair with Axichrom 50/100 series for optimization.
• Scale-Up Production: Consider Axichrom 100/500 or BPG™ series columns.
• Customization: Options for column material (e.g., PEEK) and connection sizes.

8. Support Services

GE Healthcare provides:

• Technical validation (e.g., column efficiency testing reports).
• Global after-sales network for spare parts and maintenance.

Note: Specifications may vary. For detailed parameters or application support, consult GE-authorized distributors.