Device built-in

Thermo Scientific™ Vanquish™ Ultra-High Performance Liquid Chromatography (UPLC) System Introduction

1. Overview

The Thermo Scientific™ Vanquish™ Ultra-High Performance Liquid Chromatography (UPLC) system is a high-performance liquid chromatography (HPLC) platform developed by Thermo Fisher Scientific, designed for high-resolution, high-sensitivity, and high-throughput analysis. Equipped with advanced fluidics, detection technology, and intelligent software, this system is widely used in pharmaceuticals, biotechnology, food safety, environmental monitoring, and scientific research, significantly improving analytical efficiency and data reliability.

2. Core Technical Features

(1) Ultra-High Performance Separation

The Vanquish UPLC utilizes an optimized high-pressure pump system (up to 1500 bar) combined with small-particle columns (e.g., 1.7 µm or smaller), greatly enhancing separation efficiency and resolution while reducing analysis time, making it ideal for rapid analysis of complex samples.

(2) Intelligent Temperature Control System

• Precise Column Oven Control: Wide temperature range (4°C–110°C) with minimal fluctuation (±0.1°C), ensuring retention time stability and reproducibility.
• Cooled Autosampler: Suitable for stable storage of biological samples (e.g., proteins, peptides) to prevent degradation.

(3) High-Sensitivity Detectors

The Vanquish series offers multiple detector options, including:

• Diode Array Detector (DAD): Full-wavelength scanning for simultaneous multi-component detection.
• Fluorescence Detector (FLD): High sensitivity for trace-level analysis.
• Mass Spectrometry Compatibility: Seamless integration with high-resolution mass spectrometers (e.g., Orbitrap, Q Exactive) for precise qualitative and quantitative analysis.

(4) Intelligent Autosampler System

• Ultra-Low Carryover Design: Cross-contamination <0.005%, suitable for sequential analysis of samples with large concentration differences.
• High-Throughput Mode: Supports 96/384-well plate autosampling, meeting the demands of large-scale testing in drug screening, metabolomics, and more.

(5) Modular Design

Users can flexibly configure pumps, autosamplers, column ovens, and detectors based on experimental needs, with options for future upgrades and expansions.

3. Application Areas

(1) Pharmaceutical Research & Quality Control

• Pharmacokinetics (PK/PD) Studies: High-sensitivity detection of drug concentrations in blood.
• Impurity Analysis: High-resolution separation of drug degradation products or synthetic byproducts.
• Biopharmaceuticals: Purity and stability analysis of monoclonal antibodies, ADCs (antibody-drug conjugates), etc.

(2) Life Science Research

• Proteomics/Metabolomics: Coupled with mass spectrometry for in-depth analysis of complex biological samples.
• Nucleic Acid Analysis: Purity testing of oligonucleotides, RNA/DNA.

(3) Food Safety & Environmental Monitoring

• Pesticide Residues & Additive Detection: High-sensitivity quantification of harmful substances in food.
• Environmental Pollutant Analysis: Such as polycyclic aromatic hydrocarbons (PAHs), microplastics, etc.

4. Software & Data Management

The Vanquish system is paired with Chromeleon™ Chromatography Data System (CDS), offering:

• Intelligent Method Development: Automated optimization of gradients, flow rates, and other parameters.
• Regulatory Compliance: Meets FDA 21 CFR Part 11, GMP/GLP requirements, ensuring data integrity.
• Cloud Collaboration: Supports remote monitoring and data sharing.

5. Key Advantages

• Higher Efficiency: Up to 3–5x faster analysis than traditional HPLC, with 90% reduction in solvent consumption.
• Higher Sensitivity: Detection limits as low as pg-level, suitable for trace analysis.
• Greater Reliability: Intelligent diagnostics and warning functions minimize system failure risks.
• Broader Compatibility: Seamless integration with Thermo Fisher’s mass spectrometry and spectroscopy platforms for comprehensive analytical solutions.

6. Conclusion

With its exceptional separation performance, intelligent operation, and broad applicability, the Thermo Scientific™ Vanquish™ UPLC system has become a cornerstone of modern analytical laboratories. Whether in drug development, biological research, or environmental and food safety testing, the Vanquish UPLC delivers efficient, precise, and reliable analytical solutions, helping scientific and industrial users overcome technical challenges and enhance data quality and productivity.