Device built-in

Introduction to Molecular Devices SpectraMax 190 and 384 Full-Wavelength Absorbance Microplate Readers

Molecular Devices' SpectraMax 190 and 384 are high-performance absorbance microplate readers specifically designed for nucleic acid quantification, protein analysis, and enzymatic assays in life science research and drug development.

1. SpectraMax 190 Absorbance Microplate Reader

The SpectraMax 190 is optimized for 96-well plates, featuring a full 190-850nm UV-Vis wavelength range. Utilizing monochromator technology with 1nm wavelength increments, it eliminates the need for filter changes while ensuring high-precision detection. Key features include:

• High-precision optics: 2nm spectral bandwidth, ±2.0nm wavelength accuracy, and linear detection range up to 4.0 OD, guaranteeing reliable results for nucleic acid (A260/A280) and protein assays (Bradford, BCA).
• PathCheck™ technology: Automatically converts microplate readings to 1cm cuvette-equivalent pathlength, eliminating variations caused by liquid volume differences.
• Temperature control & automation: Maintains temperatures from ambient to 45°C with optional shaking, and integrates with StakMax stackers for high-throughput workflows.

2. SpectraMax 384 Absorbance Microplate Reader

Building on the 190's capabilities, the SpectraMax 384 supports 384-well plates and extends the wavelength range to 190-1000nm for higher throughput applications. Its advanced features include:

• Multi-channel detection & rapid reading: 9-channel optical system (8 wells + 1 cuvette) enables 5-second scans for 96-well plates and 16 seconds for 384-well plates.
• Dual-mode design: Compatible with microplates, cuvettes, and test tubes, supporting spectral scanning, kinetic, and endpoint assays.
• Ultra-low volume option: Optional SpectraDrop™ module requires only 2μL per sample (64 samples total), detecting dsDNA at sensitivities as low as 2ng/μL.

Applications & Software Support

Both instruments are ideal for ELISA, cell proliferation/toxicity (MTT), microbial growth analysis, and enzyme kinetics studies. The SoftMax® Pro Software provides advanced data analysis (4PL curve fitting), compliance tools (FDA 21 CFR Part 11), and automation integration.

Conclusion: The SpectraMax 190 excels in routine 96-well plate applications, while the 384 model offers superior throughput and wavelength range. Both deliver the precision and flexibility required for modern absorbance-based assays.