Device built-in

Introduction to Molecular Devices FlexStation 3 Multimode Microplate Reader

The FlexStation 3 is an integrated multimode microplate detection platform developed by Molecular Devices, combining optical detection with automated liquid handling capabilities. It is widely used in life science research, drug screening, and cell biology applications.

Core Features & Detection Modes

1. Multimode Detection Capabilities

   • Absorbance: Supports a wavelength range of 200–1000 nm, suitable for protein quantification (e.g., BCA assay), enzyme activity analysis, and microbial growth monitoring.
   • Fluorescence Intensity (FI): Excitation range 250–850 nm, emission range 360–850 nm, ideal for nucleic acid labeling (e.g., SYBR Green) and fluorescent reporter genes (e.g., GFP).
   • Luminescence: No excitation light required, optimized for reporter gene assays (e.g., Luciferase) and ATP detection.
   • Time-Resolved Fluorescence (TRF) & Fluorescence Polarization (FP): Used in drug screening and protein interaction studies.

2. Automated Liquid Handling System
   Equipped with an 8- or 16-channel pipettor (1–200 μL, minimum 1 μL resolution), enabling precise reagent dispensing and real-time kinetic measurements (e.g., calcium flux, GPCR activity).

Technical Advantages

• Flexible Optics: Dual monochromators eliminate the need for filters, with 1 nm wavelength adjustment for optimized fluorescence detection.
• High Sensitivity & Precision: Fluorescence sensitivity down to 2 pM fluorescein, absorbance accuracy of ±0.005 OD, and superior luminescence signal-to-noise ratio.
• Temperature Control & Compatibility: Maintains temperatures from ambient to 45°C, compatible with 6- to 384-well plates (flat-, round-, or V-bottom).

Applications

The FlexStation 3 excels in drug discovery (e.g., inhibitor screening, calcium signaling), cell function studies (e.g., membrane potential, ROS detection), and molecular interaction assays. Its SoftMax Pro software enables advanced data analysis and compliance with GMP/GLP standards.

Conclusion: With its multiplex detection, automation, and high-throughput design, the FlexStation 3 is an ideal solution for complex biological assays in modern laboratories.