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Thermo Scientific™ Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ Mass Spectrometer - Product Introduction

Product Overview
The Thermo Scientific™ Q Exactive™ HF-X is an ultra-high resolution hybrid quadrupole-Orbitrap™ mass spectrometer specifically designed for proteomics, biopharmaceutical and metabolomics research. This system combines high-performance quadrupole precursor ion selection with an ultra-high field Orbitrap™ mass analyzer, achieving breakthrough improvements in sensitivity, resolution and scanning speed. It was awarded the "2017 Medicine Maker Innovation Award" by The Medicine Maker.

Core Technological Advantages

1. Ultra-High Sensitivity

   • Features a new large-bore ion transfer tube and electrodynamic ion funnel, delivering 2-3 times higher sensitivity than previous generations (detection limit down to fmol level)
   • 8x improved signal-to-noise ratio, particularly suitable for monoclonal antibody mass determination and glycosylation analysis

2. Exceptional Resolution

   • Orbitrap™ mass analyzer achieves resolution up to 240,000 FWHM (at m/z 200), capable of distinguishing 6 mDa mass differences
   • Mass accuracy <1 ppm, supporting isotopic fine structure analysis

3. High-Speed Scanning Technology

   • Ultra-fast scanning at 40 Hz with APD (Advanced Peak Determination) algorithm increases protein identification throughput by 42%
   • 30-minute analysis equivalent to 1-hour work on conventional instruments

Key Applications

• Proteomics: Achieves world-record identification of 1,100 unique peptides per minute
• Biopharmaceuticals: ADC drug characterization, PTM site analysis
• Metabolomics: Detection of low-abundance metabolites in complex matrices

System Configuration

• Ion source: Dual-mode nano/micro electrospray ionization source
• Software: Proteome Discoverer 2.2 and Compound Discoverer platforms
• ISO certified with FDA 21 CFR Part 11 compliant data

This instrument has become core equipment at leading institutions including Tsinghua University's Protein Research Center, with its technological innovations continuously advancing disease research and drug discovery.