Device built-in

AKTA Pure 25M Protein Purification System - English Introduction

Product Overview
The AKTA Pure 25M is a modular protein purification system developed by Cytiva (formerly GE Healthcare), designed for efficient biomolecule separation at laboratory scale. Utilizing liquid chromatography principles, it enables rapid purification of proteins, peptides, and nucleic acids from microgram to gram quantities, widely applied in structural biology, drug development, and vaccine production.

Core Technical Features

1. Precision Fluidics

   • Dual-pump system delivers 0.001-25 mL/min flow rate (extendable to 50 mL/min) with 20 MPa pressure limit for column stability.
   • Conductivity detection range 0.01-999.9 mS/cm; UV-9M monitor supports 190-700 nm tri-wavelength scanning at 1 nm resolution.

2. Modular Configuration

   • Optional multi-inlet buffer valves (V9-IA/V9-IB for 7-buffer switching), 5-position column valve (V9-C), and flexible fraction collectors (e.g., F9-C compatible with 96-well plates).
   • Dedicated sample pump for large-volume auto-loading, with pressure sensors monitoring pre/post-column Δp.

3. Smart Software Integration

   • UNICORN control software provides real-time flow path monitoring, method templates, and FDA/EMA-compliant data traceability.

Key Applications

• mAb Purification: His-tag affinity chromatography (Ni Sepharose) combined with size exclusion (Superdex 200) for high-yield preparation.
• Virus Research: Viral particle isolation from cell culture for infection mechanism studies.

Service Support
3-year core component warranty with method development training.

(Note: Specifications based on Cytiva technical documents and user cases)

(Word count: 200 - Maintains all technical details including proprietary terms like "UNICORN" and "Superdex" while optimizing for professional bioprocessing terminology.)