Device built-in

SCIEX 6600 TripleTOF High-Resolution Mass Spectrometry System Introduction

The SCIEX 6600 TripleTOF is a globally leading quadrupole-time-of-flight high-resolution mass spectrometry system that perfectly combines the selectivity of triple quadrupole technology with the high resolution of time-of-flight mass spectrometry. Featuring innovative SWATH® Acquisition technology, this system delivers ultra-high sensitivity and precise mass measurement capabilities for proteomics, metabolomics, and biomarker discovery research.

Core Technical Features

The 6600 TripleTOF system employs a quadrupole-time-of-flight tandem configuration, comprising three core modules:

1. Ion Source System:

• Standard Turbo V™ ion source supporting both ESI and APCI ionization modes
• Innovative ion beam focusing technology significantly improves ion transmission efficiency
• Ion source temperature up to 700°C for challenging compounds

2. Mass Analyzer:

• First quadrupole (Q1) for precursor ion selection (mass range: 5-2,250 Da)
• Second quadrupole (Q2) as collision cell with adjustable collision energy (0-100 eV)
• Third time-of-flight analyzer (TOF) providing >40,000 FWHM resolution

3. Detection System:

• Multi-channel plate detector (MCP) with acquisition speed up to 100 spectra/sec
• Dynamic range >5 orders of magnitude for simultaneous detection of high/low abundance components

Performance Advantages

The 6600 TripleTOF offers several groundbreaking technologies:

1. SWATH® Acquisition Technology:

• Enables data-independent acquisition (DIA), obtaining MS/MS data for all components in a single injection
• Increases compound coverage by 5-10x compared to traditional DDA mode
• Supports 25 variable SWATH windows for optimized sensitivity across mass ranges

2. Ultra-High Mass Accuracy:

• Mass error <2 ppm enables elemental composition determination
• Real-time mass calibration ensures long-term stability
• Isotopic pattern fit >90% improves compound confirmation reliability

3. Exceptional Sensitivity:

• Full scan mode detection limit at fg level (reserpine)
• MS/MS mode detection limit <1 pg (on-column)
• Dynamic range >10⁵ for trace analysis in complex matrices

Key Application Areas

With its outstanding performance, the 6600 TripleTOF has become the gold standard in:

• Proteomics: Large-scale protein identification, post-translational modification analysis
• Metabolomics: Global small molecule analysis, metabolic pathway elucidation
• Pharmaceutical Research: Drug metabolite identification, impurity analysis
• Food Safety: Pesticide screening, adulterant detection
• Environmental Analysis: Monitoring emerging contaminants (PPCPs, EDCs)

In clinical proteomics research, the 6600 TripleTOF with SWATH technology can identify >4,000 plasma proteins in a single 30-minute analysis. For metabolomics applications, the system simultaneously detects >1,000 metabolites in polarity switching mode with mass error <1 ppm.

The SCIEX 6600 TripleTOF system represents the pinnacle of high-resolution mass spectrometry technology. Its innovative SWATH acquisition combined with ultra-high resolution capabilities opens new avenues for systems biology research.