Device built-in

Bruker timsTOF HT Mass Spectrometry System Introduction

The Bruker timsTOF HT represents Bruker's next-generation high-performance ion mobility mass spectrometry system, specifically designed for high-throughput, in-depth 4D-omics research. Incorporating fourth-generation TIMS-XR trapped ion mobility separation technology and a 14-bit digitizer, this system demonstrates exceptional performance in proteomics, metabolomics, and spatial omics applications.

Core Technical Features

The timsTOF HT features three major technological breakthroughs:

1. Fourth-Generation TIMS-XR Separation Technology:

• Utilizes novel XR cell design with 3x improved dynamic range, identifying >100,000 unique peptides (1% FDR) within 60-minute gradients
• Combines with 14-bit Digitizer technology to achieve quantitative CV <5% for high-precision quantitative proteomics
• Innovative ion focusing path design significantly reduces chemical noise and improves signal-to-noise ratio

2. High-Throughput Analysis Capability:

• Supports high-throughput mode analyzing 50-200 samples per day (SPD) while maintaining ultra-high stability
• Through dia-PASEF technology, achieves deep coverage from microgram-level samples, quantifying >550 plasma proteins in 30-minute gradients
• Integrates with CellenONE PICO liquid dispensing system to advance single-cell proteomics (SCP) applications

3. Intelligent Data Processing:

• Incorporates PaSER64 real-time data streaming analysis system for "acquisition-to-analysis" workflow
• TIMS DIA-NN module supports CCS value-assisted analysis to improve identification reliability
• Compatible with Spectronaut and other software for multiple acquisition mode data analysis

Performance Advantages

The timsTOF HT excels in several key performance metrics:

1. Unparalleled Sensitivity:

• Identifies >100,000 unique peptides from 1μg protein sample within 60-minute gradients
• In plasma proteomics, quantifies 578 proteins (804 peptides) in 30-minute gradients with >99% data completeness
• For tissue samples, identifies 4,700 proteins from 800ng injection across 5 orders of magnitude

2. Broad Application Scope:

• Spatial Proteomics: Detects 3,500-6,500 proteins in 0.5mm² samples, supporting FFPE sample analysis
• Liquid Biopsy: Optimized for unbiased plasma proteomics and biomarker discovery
• Clinical Translation: Supports large cohort studies for precision medicine requirements

3. Exceptional System Stability:

• Continuously analyzes thousands of samples without cleaning while maintaining stable performance
• Excellent mass axis stability ensures long-term quantitative accuracy
• Unique instrument design guarantees data reliability during high-throughput operation

Through its innovative ion mobility separation technology and intelligent data analysis platform, the Bruker timsTOF HT elevates 4D-omics research to new heights. Its outstanding throughput, depth, and stability make it particularly suitable for cutting-edge fields including clinical proteomics, spatial omics, and translational medicine research.