Device built-in

Thermo Scientific Orbitrap Exploris 480 High-Resolution Mass Spectrometry System

The Thermo Scientific Orbitrap Exploris 480 represents a new generation of hybrid ultra-high-resolution mass spectrometry systems from Thermo Fisher Scientific, combining Orbitrap electrostatic trap and linear ion trap dual mass analyzers. Specifically designed for proteomics, metabolomics, and biopharmaceutical research, this system delivers exceptional resolution (480,000 @ m/z 200) while significantly improving scan speed (40Hz) and sensitivity (capable of detecting 50amol trace molecules), making it an ideal analytical platform for translational medicine research and precision medicine.

Core Technical Features

The Orbitrap Exploris 480 features a modular design with three innovative components:

1. Ion Source System:

• Standard nanoESI and APCI ion sources supporting positive/negative ion mode switching (1.4Hz)
• Innovative segmented quadrupole (AQT technology) with minimum isolation window of 0.4Da
• Patented StepWave ion focusing technology providing 10x signal-to-noise ratio improvement

2. Dual Mass Analyzers:

• Orbitrap electrostatic trap: Resolution 480,000 @ m/z 200, mass range m/z 40-6000 (extendable to 8000)
• Linear ion trap (LIT): Supports 20Hz rapid MS/MS scanning
• New C-Trap and HCD collision cell supporting multiple fragmentation modes (HCD/CID/ETD)

3. Intelligent Detection System:

• EASY-IC internal standard real-time correction with mass accuracy <1ppm (internal standard)
• Dynamic range >5,000:1 for wide concentration sample analysis
• Parallel detection technology enables simultaneous high-resolution and rapid scanning

Performance Advantages

The system incorporates several groundbreaking technological innovations:

1. Ultra-High Throughput Analysis:

• Identifies >6,000 proteins in a single 60-minute LC-MS/MS analysis
• TurboTMT technology increases 11-plex labeling quantitative throughput by 3x
• BoxCar DDA/DIA technology extends dynamic range by 10x

2. Precise Quantification Capabilities:

• SureQuant targeted quantitative workflow enables PRM analysis without retention time windows
• Label-based quantification (TMT/SILAC) CV<5%, label-free quantification reproducibility >90%
• Supports single-cell level proteomics analysis (2,000+ proteins identified from 10 cells)

3. Exceptional Stability:

• 50% smaller footprint than previous generations with single turbo molecular pump
• 6-stage vacuum system design extends maintenance intervals by 30%
• <1ppm mass drift during 72-hour continuous operation

Application Fields

The Orbitrap Exploris 480 demonstrates outstanding performance across multiple cutting-edge research areas:

• Proteomics: Achieves ultra-deep coverage identifying >10,000 proteins in single experiments
• Post-Translational Modifications: Precisely locates phosphorylation, ubiquitination and other modification sites with fmole-level sensitivity
• Biopharmaceuticals: Achieves 57%/36% sequence coverage for light/heavy chains in intact antibody drug analysis
• Clinical Translation: Developed COVID-19 antigen detection method with 98% sensitivity
• Metabolomics: Simultaneously detects >1,000 metabolites with <3ppm mass error

Key Technical Specifications Summary

Through its intelligent data acquisition framework and revolutionary hardware design, the Orbitrap Exploris 480 perfectly combines high-throughput analysis with ultimate performance, establishing itself as a benchmark platform for modern omics research and biopharmaceutical development. Its exceptional stability and user-friendliness make it particularly suitable for high-throughput laboratories requiring long-term operation, providing scientists with powerful tools to explore molecular mechanisms of life.