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Thermo Scientific Orbitrap Fusion Lumos Mass Spectrometer (2015 Edition) Introduction

The Thermo Scientific Orbitrap Fusion Lumos, launched in 2015, is a high-performance tribrid mass spectrometry system specifically designed for proteomics and structural biology research. This system innovatively integrates Electron Transfer Dissociation (ETD) technology, enabling deep protein sequencing and post-translational modification analysis without requiring liquid chromatography coupling, making it a vital tool for protein research at that time.

Core Technical Features

The Fusion Lumos system employs a tribrid mass analyzer design, comprising three key modules:

1. Ion Source System:

• Standard nanoESI ion source optimized for direct infusion
• Advanced ion focusing technology provides 5x sensitivity improvement over previous generations
• Temperature range: 50-300°C for protein sample analysis
• Patented ion beam focusing technology minimizes sample loss

2. Mass Analyzer:

• Quadrupole mass selector (Q1) with mass range: 50-4000 m/z
• Orbitrap electrostatic trap with resolution up to 500,000 FWHM
• Linear ion trap (LTQ) supporting rapid MSn analysis
• Electron Transfer Dissociation (ETD) source for gentle protein fragmentation

3. Detection System:

• Dual detector design (Orbitrap and ion trap detectors)
• Mass accuracy <1 ppm (internal standard), <3 ppm (external standard)
• Dynamic range >10⁵ for wide concentration range sample analysis

Performance Advantages

The Fusion Lumos system offers several groundbreaking technological advantages:

1. Electron Transfer Dissociation (ETD) Technology:

• Preserves labile post-translational modifications (phosphorylation, glycosylation)
• Enables protein C-terminal sequencing
• Improves sequence coverage by 30-50% compared to CID/HCD

2. Ultra-High Resolution and Mass Accuracy:

• Maximum resolution of 500,000 FWHM (m/z 200)
• Mass error <1 ppm (internal standard) for precise protein identification
• Isotopic pattern fit >95% enhances protein identification reliability

3. Direct Infusion Analysis Capability:

• Analyzes protein mixtures without LC separation
• Identifies 50+ proteins in a single infusion
• Particularly suitable for purified protein sample analysis

Key Applications

With its exceptional ETD performance, the Fusion Lumos system excels in:

• Protein Sequencing: De novo sequencing of intact proteins, C-terminal sequencing
• Post-Translational Modification Analysis: Localization of phosphorylation, glycosylation, acetylation sites
• Protein-Ligand Interactions: Non-covalent complex structure studies
• Antibody Characterization: Sequence coverage and modification analysis of intact monoclonal antibodies
• Proteomics: Deep coverage analysis of simple mixtures

In antibody drug research, the Fusion Lumos system achieves >90% sequence coverage for monoclonal antibodies in a single ETD analysis. For phosphoprotein analysis, it precisely locates multiple adjacent phosphorylation sites.

The Thermo Scientific Orbitrap Fusion Lumos represented the forefront of protein mass spectrometry technology in 2015. Its innovative ETD technology and direct infusion capability provide unique solutions for protein structure research, making it an ideal tool for proteomics and structural biology studies.