Device built-in

Comprehensive Analysis of Waters Xevo G2-XS QTof Quadrupole-Time-of-Flight High-Resolution Mass Spectrometry System

The Waters Xevo G2-XS QTof is a high-performance quadrupole-time-of-flight liquid chromatography-mass spectrometry system that perfectly combines quadrupole mass selector with time-of-flight mass analyzer, delivering ultra-high resolution and accurate mass measurement capabilities for complex sample analysis. This system has become a premium research platform in proteomics, metabolomics, environmental analysis, food safety, and other fields.

System Configuration and Core Technologies

The Xevo G2-XS QTof system employs an innovative quadrupole-time-of-flight tandem configuration, comprising three core modules:

1. Ion Source System:

• Standard UniSpray ion source compatible with multiple ionization modes (ESI, APCI)
• Advanced ion focusing technology provides 10x sensitivity improvement over conventional designs
• Temperature range: 100-600°C for diverse compound analysis
• Patented StepWave ion optics system minimizes matrix interference

2. Mass Analyzer:

• Mass range: 20-80,000 Da with resolution >40,000 FWHM
• Quadrupole mass selector (Q1) enables precise precursor ion selection
• Time-of-flight analyzer (TOF) provides high-resolution mass measurement
• Adjustable collision energy (0-120 eV) supports multi-stage MS analysis

3. Detection System:

• Multi-channel plate detector (MCP) with acquisition speed up to 50 spectra/sec
• Mass accuracy <2 ppm RMS (internal standard), <5 ppm RMS (external standard)
• Dynamic range >5 orders of magnitude for wide concentration range sample analysis

Performance Advantages and Innovative Features

The Xevo G2-XS QTof system offers several groundbreaking technological advantages:

1. Ultra-High Resolution and Mass Accuracy:

• Resolution >40,000 FWHM distinguishes compounds with minimal mass differences
• Mass error <2 ppm (internal standard) enables elemental composition determination
• Isotopic pattern fit >90% improves compound confirmation reliability

2. Data-Independent Acquisition Technology:

• Supports MSE and HDMSE data acquisition modes
• Simultaneously obtains MS and MS/MS data for all components in a single injection
• Improves compound coverage by 5-10x compared to traditional DDA mode

3. Intelligent Data Processing:

• Includes Progenesis QI and UNIFI Scientific Information System
• Enables automated metabolite annotation and pathway analysis
• Seamless integration with ProteinLynx Global Server for proteomics big data processing

Key Application Areas

With its exceptional performance, the Xevo G2-XS QTof system excels in:

• Proteomics: Large-scale protein identification, post-translational modification analysis
• Metabolomics: Global small molecule analysis, novel biomarker discovery
• Environmental Analysis: Screening and identification of emerging contaminants (PPCPs, EDCs)
• Food Safety: Pesticide screening, adulterant detection, food authenticity verification
• Biopharmaceuticals: Antibody drug characterization, impurity analysis

In proteomics research, the Xevo G2-XS QTof with MSE technology can identify >4,000 proteins in a single 30-minute analysis. For metabolomics applications, the system simultaneously detects >1,000 metabolites in polarity switching mode with mass error <1 ppm.

The Waters Xevo G2-XS QTof represents the forefront of high-resolution mass spectrometry technology. Its innovative ion optics design and intelligent data processing capabilities provide powerful tools for systems biology research, making it the ideal choice for modern research laboratories.