Device built-in

Agilent Prep LC 1260 Infinity Preparative Liquid Chromatography System – English Introduction

The Agilent Prep LC 1260 Infinity is a high-performance preparative liquid chromatography system developed by Agilent Technologies, designed for the purification of samples ranging from milligrams to grams. It is widely used in drug development, protein purification, natural product isolation, and other fields. Combining modular design, precise control, and bioinert flow paths, this system delivers efficient and reliable solutions for complex sample preparation.

1. Core Performance & Modular Design

The system features a modular architecture, allowing flexible configurations such as quaternary pumps or integration with multiple detectors (e.g., UV, ELSD). Its pump module supports pressures up to 600 bar with a flow rate range of 0.05–100 mL/min, compatible with preparative columns (9.4–30 mm ID) for seamless scaling from analytical to preparative applications. The adjustable delay volume (down to 120 μL) minimizes peak broadening and fraction cross-contamination, ensuring high purity and recovery.

2. Bioinert Flow Path & Purification Capabilities

For biomolecules (e.g., antibodies, proteins), the system offers a fully inert flow path to prevent metal-induced degradation. It includes a refrigerated fraction collector (4–40°C) for temperature-sensitive compounds. Advanced delay sensor technology enables real-time fraction collection calibration, with multiple triggering modes (time, peak shape, or MS signal) for precise isolation. Additionally, the system supports high-throughput collection in microplates (4×96-well) or test tubes (up to 216 tubes).

3. Smart Features & Compliance

Real-time data processing via CAN bus and Agilent’s Buffer Advisor Software optimizes pH and solvent conditions, simplifying ion-exchange workflows. The system complies with GLP standards, featuring Early Maintenance Feedback (EMF), electronic logs, and 21 CFR Part 11-compliant software for pharmaceutical and QA/QC applications.

4. Applications

Ideal for peptide synthesis purification, natural product isolation, and biopharmaceutical process development, the system’s high-sensitivity detectors (e.g., DAD and ELSD) accommodate compounds with no UV absorption.

Conclusion

The Agilent Prep LC 1260 Infinity excels in modularity, precision, and biocompatibility, making it an ideal tool for preparative purification and complex sample analysis.