Device built-in

Illumina HiSeq 1500 Sequencing System Introduction

Product Overview

The HiSeq 1500 is a high-throughput next-generation sequencing (NGS) system developed by Illumina (USA). As an early model in the HiSeq series, it utilizes the classic Sequencing-by-Synthesis (SBS) technology and supports single-flow cell operation, making it suitable for medium-scale genomic sequencing projects. Launched in 2010, the HiSeq 1500 shares the same technology platform as the HiSeq 2500 but is limited to single-flow cell configuration, offering a cost-effective solution for laboratories balancing throughput and budget constraints.

Core Technical Features

1. Dual-Run Modes for Flexibility

   • High-Output Mode: Generates up to 300 Gb of data per run (2×100 bp read length) in approximately 8.5 days, ideal for large-scale sequencing applications such as whole-genome and exome sequencing.
   • Rapid Run Mode: Accelerates workflow with on-board cluster generation, delivering 60 Gb of data (2×100 bp) in just 27 hours, suitable for clinical or urgent research projects requiring fast results.
   • Uses reversible terminator-based SBS chemistry, ensuring high base-call accuracy (≥80% of bases above Q30 for 2×100 bp reads) and minimizing homopolymer sequencing errors.

2. Modular Design

   • Each flow cell contains 8 lanes, allowing independent sample loading for multiplexed sequencing.
   • Compatible with Illumina’s standard library prep kits (e.g., TruSeq, Nextera), supporting diverse applications, including whole-genome, transcriptome, and targeted sequencing.

3. Data Quality & Efficiency

   • Generates up to 3 billion paired-end reads per run (High-Output mode), meeting the demands of medium-throughput research.
   • Integrates with BaseSpace cloud computing for real-time data analysis and storage, streamlining bioinformatics workflows.

Typical Applications

• Human Genomics: Achieves 30× coverage whole-genome sequencing in Rapid Run mode within 27 hours.
• Clinical Diagnostics: Supports non-invasive prenatal testing (NIPT) and cancer mutation screening, meeting clinical-grade data standards.
• Microbiome & Transcriptomics: Ideal for 16S rRNA sequencing and mRNA expression profiling.

Market Positioning & Limitations

The HiSeq 1500 was a cost-effective and reliable workhorse for mid-to-high-throughput sequencing but has since been succeeded by the HiSeq 2500/4000 and NovaSeq series. Key limitations include:

• Single-flow cell configuration, limiting throughput compared to the HiSeq 2500’s dual-flow cell setup.
• Shorter read lengths (max 2×150 bp), posing challenges for complex genome assembly.

Note: The HiSeq 1500 is now discontinued, with second-hand units priced between ¥2M–5M RMB, making it a viable option for budget-conscious research institutions.