Device built-in

Akta Pure 25L Protein Purification System – English Introduction

Product Overview

The Akta Pure 25L is a pilot-scale protein purification system developed by Cytiva (formerly GE Healthcare Life Sciences), designed for process scale-up and bioprocessing development. It combines high-precision automation with modular flexibility, making it ideal for the purification of antibodies, vaccines, recombinant proteins, and other biomolecules, supporting workflows from research to small-scale production.

Key Features

1. Scalable & Flexible
   The system supports up to 25L sample loading and is compatible with various chromatography techniques, including size exclusion (SEC), ion exchange (IEX), and affinity chromatography (e.g., His-tag purification). Its modular design allows customization with multi-wavelength UV detectors, conductivity/pH monitors, and automated fraction collectors.

2. Smart Control & Compliance
   Equipped with UNICORN control software, it offers an intuitive interface for method development, optimization, and data tracking. The software complies with 21 CFR Part 11, ensuring data integrity in GMP-regulated environments.

3. High Precision & Reliability
   Features high-accuracy pumps and dynamic mixers, with a flow rate range of 0.1–100 mL/min and a pressure limit of 5 MPa, ensuring reproducible chromatography runs. Built-in bubble sensors and leak alarms enhance operational safety.

4. Applications
   Widely used for monoclonal antibody purification, viral vector concentration, membrane protein extraction, and more—ideal for process validation before pilot-scale production.

Technical Specifications

• Sample Loading Capacity: 25L (expandable)
• Flow Rate Range: 0.1–100 mL/min
• Detection Wavelength: Standard 280 nm (multi-wavelength optional)
• Compatible Resins: Pre-packed or custom columns (e.g., HiTrap, Superdex series)

Conclusion

The Akta Pure 25L stands out for its automation, precision, and scalability, making it a core system for biopharmaceutical process development, efficiently bridging the gap between lab research and production.