Device built-in

BD FACSMelody Flow Cytometry Cell Sorter Introduction

System Overview

The BD FACSMelody is an intelligent flow cytometry cell sorting system developed by BD Biosciences, designed to simplify complex cell sorting workflows. As a benchtop instrument, it combines BD's proven FACSAria platform technology with innovative automation features, enabling even novice users to master operation within 2 hours. Launched in China in 2016, the system significantly lowers the technical barrier of traditional cell sorters, which typically require 5 days of training.

Core Technical Features

1. Smart Operation:

   • Equipped with FACSChorus software, reducing operational steps by 55% through guided menus
   • Automatically performs fluidic calibration, drop delay calculation, and other complex settings, enabling sorting within 12-17 minutes after startup
   • Built-in 60-day compensation-free function ensures consistent results across users

2. Superior Sorting Performance:

   • Features a fixed optical path with a quartz cuvette for fg-level sensitivity
   • Supports 9-color multiparameter detection, identifying low-expression and rare cell subsets (purity >98.8%)
   • Sorting speed up to 1,000 cells/second, compatible with 96/384-well plate sorting

3. Biosafety Design:

   • Optional Class II Type A2 biosafety cabinet complies with NSF49 and EN12469 standards
   • Integrated aerosol control system provides dual protection for operators and samples

Applications

• Cancer Research: High-purity sorting of EpCAM+ tumor cells for single-cell sequencing
• Immunology: Isolation of rare immune cell subsets (e.g., Tregs)
• Vaccine Development: Screening of virus-specific T cells
• Genetic Engineering: Sorting CRISPR-edited cells for animal model construction
• Drug Discovery: High-throughput screening of yeast display libraries

System Advantages

1. Enhanced Efficiency: Unattended automated sorting with QC system predicting laser failures
2. Cost Optimization: Fixed flow cell design reduces consumables, maintenance time shortened to 3 minutes
3. Flexible Configuration: Six configurations available for diverse needs, from basic research to clinical translation

By making advanced technology "user-friendly," the BD FACSMelody brings high-performance cell sorting to standard laboratories. Currently used in top research institutions worldwide for cancer, immunology, virology, and other cutting-edge fields, its accessible price of ¥235,000 (2024 quote) further lowers the adoption barrier.