Device built-in

AB Sciex QTRAP 4500 with Nexera HPLC LC-MS/MS System Introduction

System Overview

The AB Sciex QTRAP 4500 coupled with Nexera HPLC system is a high-performance analytical platform jointly developed by SCIEX and Shimadzu, integrating the QTRAP 4500 hybrid triple quadrupole-linear ion trap mass spectrometer with Shimadzu's Nexera ultra-high performance liquid chromatography system. This combined system delivers both ultra-high sensitivity quantitative capabilities and superior separation performance, making it widely applicable in pharmaceutical R&D, food safety, environmental monitoring, and clinical research.

Core Component Technical Features

1. QTRAP 4500 Mass Spectrometer:

   • Patented QTRAP® technology with mass range of 5-2,000 Da (triple quad mode) and 50-2,000 Da (ion trap mode)
   • MRM mode sensitivity reaching 200 fg reserpine (S/N>2,000), with ion trap scanning sensitivity 10-100× higher than conventional triple quads
   • Supports 12 scanning modes including MRM, MRM³, and EPI; Turbo V ion source temperature up to 750°C
   • Equipped with AcQuRate pulse counting detector offering dynamic range >10⁶

2. Nexera HPLC System:

   • Ultra-high pressure design (130MPa) compatible with sub-2μm particle columns
   • Flow rate range 0.0001-5mL/min, injection precision 0.2-20μL
   • Low dead-volume flow path design with minimized dwell volume, supporting overlapping injections
   • SIL-30AC autosampler with carryover <0.005%

Combined System Advantages

1. Analytical Performance:

   • Enables accurate quantification of fg-level compounds in complex matrices
   • Linear ion trap provides MS/MS structural confirmation to eliminate false positives
   • 50ms polarity switching speed ideal for high-throughput screening

2. Application Fields:

   • Pharmaceuticals: Drug metabolite identification and quantification (MRM-IDA-EPI mode)
   • Food Safety: Multi-residue detection of pesticides/veterinary drugs with MRM³ technology eliminating matrix interference
   • Environment: Monitoring POPs and emerging contaminants in water/soil
   • Clinical: Newborn screening and vitamin D testing (CLIA certified)

3. Operational Convenience:

   • Unified control via Analyst® software compliant with 21 CFR Part 11
   • Modular design supports switching between ESI/APCI and other ion sources
   • System throughput exceeding 300 samples per day

This integrated system combines the "rod-trap" hybrid scanning capabilities of SCIEX QTRAP 4500 with the ultra-high separation efficiency of Nexera UHPLC, delivering an industry-leading solution for qualitative and quantitative analysis of complex samples.