Device built-in

Illumina MiniSeq Sequencing Kit Introduction

Product Overview

The Illumina MiniSeq Sequencing Kit is a specialized reagent set designed for the MiniSeq high-throughput sequencing system, employing the proven Sequencing-by-Synthesis (SBS) technology to provide a complete workflow solution from DNA library preparation to sequencing analysis. This series includes two specifications - MiniSeq Mid Output and MiniSeq High Output - supporting 30 million and 50 million reads output respectively, meeting the needs of sequencing projects at different scales. The optimized kit design makes it particularly suitable for targeted sequencing, exome sequencing, and small genome sequencing applications in small laboratories.

Core Components and Features

1. Sequencing Kit: Contains SBS chemistry reagents specifically optimized for the MiniSeq system, using four-color fluorescently labeled reversible terminators to ensure high-quality base calling. The lyophilized bead format enables room temperature shipping and 4℃ storage, significantly simplifying workflows.

2. Flow Cell: The high-density flow cell surface is modified with oligonucleotide primers, containing 250 million cluster sites for Mid Output and 400 million for High Output models. Optimized surface chemistry improves cluster generation efficiency by 15-20% while reducing reagent consumption.

3. Buffer System: Includes critical components such as cluster generation reagents, sequencing primers, and wash buffers. All components undergo stringent QC testing with <5% lot-to-lot variation, ensuring sequencing data reliability.

Technical Advantages

• High-quality data: ≥80% Q30 bases with read lengths up to 2×150bp, meeting most targeted sequencing requirements
• Flexible throughput: Mid Output kit suits 50-200 targeted sequencing samples; High Output supports 200-500 samples
• User-friendly operation: Pre-mixed reagents and automated workflows deliver data in just 12-24 hours from sample
• Cost-effective: Reduces running costs by over 50% compared to larger sequencers, ideal for small-scale projects

Application Fields

MiniSeq Sequencing Kits are widely used in:

1. Cancer research: Tumor panel sequencing, liquid biopsy, and mutation validation
2. Genetic disease testing: Single-gene disorder screening and carrier detection
3. Microbiome studies: 16S rRNA gene sequencing and microbial identification
4. Immunome profiling: TCR/BCR receptor diversity analysis
5. Agricultural genomics: Crop variety identification and molecular marker development

Usage Considerations

1. Use kits before expiration and promptly after opening
2. Store flow cells in dry conditions, avoiding freeze-thaw cycles
3. Recommended to use Illumina-certified library preparation kits
4. Perform quality control and quantification before sequencing
5. Regular system calibration and maintenance ensures optimal performance

With its reliable performance and simplified operation, the Illumina MiniSeq Sequencing Kit has become the preferred solution for targeted sequencing in small laboratories, providing strong support for precision medicine research and clinical translation applications.