Device built-in

Sapphire Biomolecular Imager Multispectral Laser Imaging System Introduction

Product Overview

The Sapphire Biomolecular Imager is a next-generation multispectral laser imaging system developed by Azure Biosystems, providing high-sensitivity, high-resolution biomolecular detection solutions for life science research through innovative multispectral laser scanning technology. This system integrates three functional modules - fluorescence imaging, chemiluminescence imaging, and stain-free imaging - to meet various experimental needs including Western blot, protein gels, and nucleic acid gels. It is widely used in molecular biology, cell biology, and drug discovery fields.

Core Technical Features

1. Multispectral Laser Scanning Technology: Equipped with six high-power solid-state lasers (488nm, 532nm, 635nm, 658nm, 685nm, and 785nm) covering common fluorescent dye excitation spectra, with excitation efficiency 10 times higher than traditional LED light sources. The system uses a highly sensitive CCD detector with quantum efficiency up to 85%, capable of detecting protein samples at fg levels.

2. Intelligent Focusing System: Patented autofocus technology precisely identifies sample position with ±50μm focusing accuracy, ensuring clear images for gels and membranes of different thicknesses. Maximum scanning resolution reaches 10μm, 5 times higher than conventional imaging systems.

3. Multi-channel Detection Capability: Supports simultaneous detection of various fluorescent dyes including Cy2, FITC, Cy3, Texas Red, Cy5, and Cy7, with spectral unmixing algorithms effectively eliminating channel crosstalk (<0.5% cross-interference in multicolor fluorescence detection).

Application Advantages

• Western Blot Detection: Chemiluminescence detection sensitivity reaches ag level with dynamic range exceeding 5 orders of magnitude, enabling simultaneous detection of multiplex fluorescently labeled protein bands
• Nucleic Acid Analysis: Detects DNA as low as 20pg, compatible with various nucleic acid stains including EB, SYBR®, and SYPRO®
• Protein Gel Imaging: Stain-free technology directly detects 1ng total protein, eliminating staining/destaining steps
• Microplate Detection: Compatible with 96-well and 384-well plates for cell fluorescence quantification and reporter gene assays

System Features

1. User-Friendly Operation: 7-inch touchscreen control with 30+ preset experimental protocols supporting one-touch imaging
2. Data Security: Complies with 21 CFR Part 11 regulations, supporting audit trails and electronic signatures
3. Expandability: Optional NIR imaging module extends applications to near-infrared fluorescence detection
4. Intelligent Analysis: Built-in Image Studio software supports automatic band recognition and quantitative analysis

With its outstanding performance and reliable operation, the Sapphire Biomolecular Imager has become the preferred imaging equipment for numerous research institutions and pharmaceutical companies, providing powerful technical support for life science research.