Device built-in

ABI 3500xl Genetic Analyzer Introduction

System Overview

The ABI 3500xl Genetic Analyzer is an upgraded 96-well high-throughput genetic analysis system developed by Applied Biosystems (USA), significantly improving detection throughput and automation level based on the classic 3500 series. Utilizing Sanger sequencing and capillary electrophoresis technologies, this FDA and CE-IVD dual-certified system is particularly suitable for large-scale genome sequencing projects and high-throughput requirements of clinical diagnostic laboratories. It has become an essential tool for NGS verification and clinical molecular diagnostics.

Core Technical Features

The system employs a next-generation 505nm solid-state laser with a 96-capillary array, supporting fully automated 96-well plate loading. The innovative dynamic temperature control system (±0.1°C accuracy) ensures stability during prolonged operation. Upgraded POP-7™ polymer matrix delivers read lengths up to 1200bp with single-base resolution. Intelligent RFID technology monitors consumable status in real-time, including capillary lifespan and buffer remaining. The 6-color fluorescence detection system provides 12-fold higher throughput than standard 3500 series.

Performance Parameters

• Throughput: Capable of analyzing over 1,000 samples within 24 hours
• Sequencing quality: Phred Q30 score ≥85%, accuracy ≥99.99%
• Operating parameters: Voltage 15kV, temperature range 18-65°C
• System specifications: 132cm(W)×81cm(D)×180cm(H), weight 320kg
• Environmental requirements: Temperature 15-30°C, humidity 20-80%, power supply 200-240V, 50/60Hz

Application Fields

The system plays critical roles in multiple domains:

• Large-scale genome projects: Whole genome sequencing verification and gap filling
• Clinical diagnostics: Genetic disease screening, tumor gene panel validation
• Pharmacogenomics: SNP genotyping and drug metabolism studies
• Agricultural genomics: Crop variety identification and molecular breeding
• Forensic databases: STR genotyping and DNA profiling

System Advantages

1. High throughput: 96-capillary parallel detection, processing 96-384 samples daily
2. High precision: Upgraded optical system improves signal acquisition sensitivity by 30%
3. Intelligent operation: Data Collection software enables unattended operation
4. Compliance: Meets CLIA, CAP and other clinical laboratory certification requirements
5. Expandability: Optional automated sample preparation modules available

Note: This system requires professional molecular laboratory environment and trained operators. Annual maintenance is recommended to ensure optimal performance.