Device built-in

SCIEX Triple TOF 6600 High-Resolution LC-MS/MS System Introduction

System Overview

The SCIEX Triple TOF 6600 is a high-performance quadrupole-time-of-flight tandem mass spectrometry (Q-TOF) system developed by AB SCIEX (now SCIEX), which perfectly combines high-resolution accurate mass measurement with rapid scanning capability. Featuring the innovative SWATH® 2.0 data-independent acquisition (DIA) technology, this system enables simultaneous qualitative exploration and quantitative analysis in a single run, providing comprehensive solutions for complex sample research.

Core Technical Features

The Triple TOF 6600 system is equipped with a third-generation orthogonal acceleration time-of-flight mass analyzer, offering a mass range of 5-40,000 Da, resolution up to 50,000 (FWHM), and mass accuracy <0.5 ppm (with internal standards). Its unique SelexION® differential ion mobility technology provides an additional separation dimension, significantly improving the resolution of isomeric compounds. The system incorporates a Turbo V™ ion source with temperatures up to 750°C, combined with QJet® ion guide technology to ensure efficient ion transmission and stable analysis.

System Advantages

1. Comprehensive data acquisition: SWATH® 2.0 technology supports up to 200 variable windows per cycle, capturing both low-abundance signals (windows as narrow as 2Da) and wide-range precursor ions
2. Ultra-high sensitivity: Column injection of 1pg reserpine achieves S/N≥1000:1 (MS mode) and S/N≥3000:1 (MS/MS mode)
3. Rapid analysis: Acquisition speeds of 25 MS spectra/second and 100 MS/MS spectra/second, perfectly matching ultra-fast LC separation
4. Intelligent data processing: Includes Analyst® TF software supporting automatic molecular formula prediction and database searching

Typical Application Fields

• Proteomics: Quantitative analysis of thousands of proteins in a single run, studying post-translational modifications and protein interaction networks
• Metabolomics: Untargeted metabolite screening and lipidomics research, compatible with specialized analysis software like XCMS plus
• Drug development: Metabolite identification, impurity analysis, and drug interaction studies
• Environmental and food safety: Screening of persistent organic pollutants (POPs) and identification of unknown additives

Service Support

SCIEX provides comprehensive technical training resources, including online courses on TripleTOF system operation and SWATH data processing. The system is ISO-certified and complies with international standards such as EPA Method 8270, making it an ideal platform for advanced laboratories conducting complex sample analysis.