Device built-in

Comprehensive Analysis of Waters Alliance 2695 HPLC System

The Waters Alliance 2695 High Performance Liquid Chromatography (HPLC) system is a classic product from Waters Corporation, renowned for its exceptional stability, high precision, and flexible configuration. Widely applied in pharmaceuticals, food, environmental, and life sciences fields, this modular system integrates advanced solvent and sample management technologies to provide reliable separation and analysis solutions for laboratories.

System Architecture and Core Technologies

At the heart of the Alliance 2695 system lies its quaternary gradient pump and 120-position autosampler separation module. The solvent management system features independent linear dual-piston drives with dual pressure sensor feedback loops, delivering pulse-free mobile phase flow with a flow rate range of 0.010-10.000 mL/min (increments of 0.001 mL/min), flow precision of 0.075% RSD, dwell volume <650μL, and maximum operating pressure of 5000 psi. The innovative online vacuum degassing technology utilizes second-generation polymer membranes to effectively eliminate bubble interference in mobile phases. Supporting quaternary gradient mixing (0.0-100.0% in 0.1% increments) with 11 gradient curve options, it meets requirements from isocratic to complex gradient elution.

The sample management system accommodates five sample trays holding up to 120 standard 2mL vials, with injection volumes from 0.1-100μL (expandable to 2000μL), injection precision <0.5% RSD, and carryover <0.1%. Programmable needle wash protocols effectively reduce cross-contamination, while the STAT function enables priority processing of urgent samples. An optional 4-40°C sample compartment temperature control module ensures stability for thermally sensitive samples.

Performance Features and Application Advantages

The Alliance 2695 system demonstrates outstanding reproducibility, with its fixed dwell volume design ensuring gradient consistency across varying pressure conditions and excellent retention time repeatability for accurate peak identification. Supporting column temperature control (ambient +5°C to 80°C) with optional heating/cooling column ovens (4-65°C), it effectively compensates for separation impacts from ambient temperature fluctuations. Through Alliance series switching valve options, automatic switching of up to six columns significantly enhances method development efficiency.

FDA-approved and pharmacopeia-listed, the system serves multiple applications: pharmaceutical content determination and impurity analysis; food safety testing for pesticide residues and illegal additives; environmental monitoring of water and soil contaminants; and life science research for qualitative/quantitative analysis of small molecule natural products and biological samples. Compatible with various detectors including PDA, UV/Vis, fluorescence, ELSD, and mass spectrometers (e.g., Quattro micro API), it meets diverse analytical needs.

Intelligent Features and Expansion Capabilities

The Alliance 2695 seamlessly integrates with Waters Empower/MassLynx software platforms for fully automated sequence analysis and data processing. Its intuitive interface simplifies method development, while QuanOptimize functionality automatically optimizes MRM channel parameters. Multiple expansion options include: GPC components for gel permeation chromatography; post-column derivatization systems integrating temperature control modules and dual reagent pumps to improve reaction efficiency; and barcode decoding modules for automated sample tracking.

Waters provides comprehensive technical support services including installation, routine maintenance, and operator training to ensure long-term stable operation. After over two decades of technological iterations, the Alliance 2695 has become an industry benchmark, its superior performance helping global laboratories address increasingly complex analytical challenges.