Recently, the Ministry of Ecology and Environment has released the *Determination of Hexabromocyclododecane (HBCD) and Tetrabromobisphenol A (TBBPA) in Water - Isotope Dilution/Liquid Chromatography-Triple Quadrupole Mass Spectrometry Method* (Draft for Comment). Excerpts of some contents are as follows: ### 1 Scope of Application This standard specifies the isotope dilution/liquid chromatography-triple quadrupole mass spectrometry method for the determination of hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) in water. This standard is applicable to the determination of α-hexabromocyclododecane, β-hexabromocyclododecane, γ-hexabromocyclododecane and tetrabromobisphenol A (TBBPA) in surface water, groundwater, domestic sewage, industrial wastewater and seawater. ### 3 Method Principle Under acidic conditions, hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) in the sample are extracted with dichloromethane. The concentrated extract is purified by a silica gel column or a composite silica gel column, then concentrated again to convert the solvent to methanol. Subsequently, the extract is determined by a liquid chromatography-triple quadrupole mass spectrometer. Qualitative analysis is conducted based on retention time and characteristic ion abundance ratio, while quantitative analysis is performed using the isotope dilution method. ### 6 Instruments and Equipment 6.1 Sampling Bottles: 1 L, brown ground-glass stoppered bottles or brown screw-cap glass bottles with polytetrafluoroethylene (PTFE)-lined caps. 6.2 Liquid Chromatography-Triple Quadrupole Mass Spectrometer (LC-MS/MS): The liquid chromatography system shall be equipped with a gradient elution function; the mass spectrometer shall be a triple quadrupole type with an electrospray ionization (ESI) source and shall have a multiple reaction monitoring (MRM) function. 6.3 Chromatographic Column: Packed with octadecylsilane-bonded silica gel (ODS), with a particle size of 1.8 μm, a column length of 100 mm, and an inner diameter of 2.1 mm. Other chromatographic columns with equivalent performance are also acceptable. 6.4 Concentration Equipment: Nitrogen evaporators, rotary evaporators, or other equipment with equivalent performance. 6.5 Separatory Funnels: 2000 mL, equipped with PTFE stopcocks. 6.6 Glass Packed Columns: Inner diameter of 15 mm～19 mm, column length of 20 cm or more. 6.7 Common laboratory instruments and equipment. ### Supplementary Notes 1. **Term Consistency & Professionalism** - Core pollutants "六溴环十二烷" and "四溴双酚A" are translated as "hexabromocyclododecane (HBCD)" and "tetrabromobisphenol A (TBBPA)" (with standard abbreviations marked for the first time), consistent with international environmental monitoring terminology (e.g., ISO, EPA standards). - Technical terms such as "液相色谱-三重四极杆质谱仪" (liquid chromatography-triple quadrupole mass spectrometer, abbreviated as **LC-MS/MS**), "电喷雾离子源" (electrospray ionization, **ESI**), and "多反应监测" (multiple reaction monitoring, **MRM**) adopt industry-universal abbreviations to ensure accuracy and readability in scientific documents. 2. **Equipment Description Accuracy** - "聚四氟乙烯衬垫/活塞" is translated as "polytetrafluoroethylene (PTFE)-lined" or "PTFE stopcocks" (PTFE is the standard English abbreviation for 聚四氟乙烯, avoiding the cumbersome full name "polytetrafluoroethylene" in repeated mentions). - "十八烷基硅氧烷键合硅胶" is译为 "octadecylsilane-bonded silica gel (ODS)" (ODS is the common abbreviation for this chromatographic packing, widely used in LC-MS methods). 3. **Sentence Structure Optimization** - For long technical clauses (e.g., Section 3 "method principle"), English sentences are split logically (e.g., using "subsequently" to connect extraction-purification-determination steps) to avoid overly complex structures, while retaining the rigor of standard specifications. - Phrases like "或其他性能相当的" are uniformly translated as "or other... with equivalent performance" to maintain consistency in the description of alternative equipment, conforming to the wording habits of international standard documents. 4. **Document Format** - Section numbers (1, 3, 6) and subclauses (6.1-6.7) are retained in the original format to ensure the structure of the standard is clear; the title of the method is italicized (*... Method*) to distinguish it as a formal standard document title.