Flow Cytometer Maintenance Guide: Essential Practices for Optimal Performance

Flow cytometers are precision instruments widely used in laboratories, and regular maintenance is crucial to extend their lifespan and ensure accurate results. Below are key maintenance recommendations:

1. Fluidic System Cleaning

• Post-run cleaning: Flush sample lines with deionized water or dedicated cleaning solution to prevent clogging.
• Monthly deep clean: Use 70% ethanol or 10% bleach (chlorine-based) to disinfect the fluidics and prevent bio-contamination.

2. Optical Component Care

• Keep lasers and lenses dust-free to maintain signal quality.
• Clean with lint-free wipes and optical-grade solution—never touch optical surfaces directly.

3. Calibration & Quality Control

• Weekly calibration: Use CS&T beads to align optics and optimize PMT voltages.
• Daily QC: Run control samples after startup to monitor performance.

4. Environmental Conditions

• Maintain stable temperature (18–25°C) and humidity (40–70%).
• Avoid vibrations/direct sunlight; check cooling fans to prevent overheating.

5. Sheath Fluid & Waste Management

• Use high-quality sheath fluid (e.g., filtered PBS) to avoid particle buildup.
• Empty waste containers regularly to prevent backflow contamination.

6. Software & Data Security

• Update instrument drivers and analysis software periodically.
• Back up data to prevent loss from system failures.

7. Maintenance Log & Professional Service

• Assign a dedicated technician to document maintenance (dates, replaced parts).
• Schedule annual professional servicing (laser alignment, fluidics inspection).

Key Benefits of Regular Maintenance:
✓ 30% longer instrument lifespan
✓ <5% CV in reproducibility
✓ 50% fewer unplanned repairs

(Follow manufacturer guidelines (e.g., BD FACSDiva, Beckman Coulter CytExpert) for model-specific protocols.)

Pro Tip: For labs running >20 samples/day, implement biweekly PMT voltage optimization to maintain sensitivity.

Emergency Contacts:

• BD Biosciences Support: [Local hotline]
• Beckman Coulter Technical Service: [Local hotline]

(Compliant with ISAC guidelines and GLP standards.)