Device built-in

Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometry System Introduction

The Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer represents the pinnacle of current mass spectrometry technology as the third-generation "tribrid" ultra-high-resolution mass spectrometer from Thermo Fisher Scientific. This system innovatively integrates three mass analyzers (QR5 segmented quadrupole, Orbitrap electrostatic trap, and linear ion trap) with multiple fragmentation modes (CID, HCD, and ETD), providing unprecedented analytical capabilities for proteomics, biopharmaceutical research, and protein higher-order structure analysis.

Core Technological Innovations

The Orbitrap Eclipse features several groundbreaking hardware upgrades:

1. QR5 Segmented Quadrupole: Utilizing hyperbolic design with isolation window as narrow as 0.4 m/z, significantly improving precursor ion selectivity and sensitivity while enhancing transmission efficiency and specificity at 0.4 m/z isolation window.

2. Real-Time Search Technology: Incorporates built-in Comet search engine for real-time database searching, substantially improving the depth and accuracy of TMT-labeled quantitative analysis while addressing the "ratio compression effect" in traditional MS2 quantification.

3. High Mass Range MSn (HMRn): Extends Orbitrap mass range to 8000 m/z, becoming the first Orbitrap instrument combining MSn capability with high m/z analysis, particularly suitable for protein-small molecule complex studies.

4. Proton Transfer Charge Reduction (PTCR) Technology: Simplifies complex spectra interpretation by reducing protein charge states through gas-phase ion-ion reactions, enhancing feasibility of top-down analysis.

Performance Specifications

The system delivers industry-leading technical parameters:

• Resolution: Up to 1,000,000 FWHM (m/z 200), capable of distinguishing minute mass differences
• Mass Accuracy: <1 ppm (internal calibration), <3 ppm (external calibration)
• Scan Speed: Orbitrap MS2 at 40 Hz, ion trap MS2 at 45 Hz
• Sensitivity: Full scan mode S/N >200:1 for 100fg reserpine
• Multistage MS: Supports up to MS10 analysis

Application Fields

The Orbitrap Eclipse demonstrates exceptional performance across multiple research areas:

1. Proteomics: Identifies >6,000 proteins in a single 60-minute analysis, with single-cell analysis identifying 1,000+ proteoforms
2. Post-Translational Modification Studies: ETD fragmentation precisely locates labile modification sites including phosphorylation and ADP-ribosylation
3. Biopharmaceuticals: Achieves 57% sequence coverage for antibody light chains and 36% for heavy chains in intact antibody analysis
4. Structural Biology: Combines HMRn with multiple fragmentation modes to simultaneously resolve protein structures and bound small molecules

With its ultra-high resolution, exceptional sensitivity, and multifunctional integration, the Orbitrap Eclipse has become the ideal platform for solving complex biological problems, providing powerful technological support for protein science research.