Device built-in

GE-Cytiva Biacore T200 Biomolecular Interaction Analysis System – English Introduction

Product Overview

The Biacore T200, developed by Cytiva (formerly GE Healthcare Life Sciences), is a next-generation high-sensitivity, label-free biomolecular interaction analysis system based on Surface Plasmon Resonance (SPR) technology. It enables precise measurement of binding kinetics (Kon/Koff), affinity (KD), and thermodynamic parameters for interactions between proteins, antibodies, nucleic acids, small-molecule drugs, and other biomolecules. Widely used in drug discovery, antibody engineering, cell signaling research, and biopharmaceutical quality control, this system provides critical insights into molecular interactions.

Key Features

1. Ultra-High Sensitivity & Precision

   • Detection limit as low as 0.1 pg/mm², capable of analyzing extremely weak binding (KD down to 10⁻¹² M) and rapid dissociation (Koff up to 20 s⁻¹).
   • Temperature control (4–45°C) supports thermodynamic parameter analysis (ΔH/ΔS), ideal for drug stability studies.

2. Fully Automated & High-Throughput Operation

   • Equipped with a 384-well autosampler, enabling unattended continuous detection for improved efficiency.
   • Features an intelligent microfluidic system, minimizing sample consumption (as low as 5 μL) and reducing experimental costs.

3. Smart Data Analysis

   • Integrated Biacore Insight software provides real-time sensorgram analysis and multiple kinetic model fittings (e.g., 1:1 binding, bivalent binding, multivalent binding).
   • Complies with FDA 21 CFR Part 11 regulations, meeting data integrity requirements in GMP/GLP environments.

4. Versatile Application Compatibility

   • Supports multiple sensor chips (CM5, NTA, SA, L1, etc.), suitable for analyzing complex samples such as membrane proteins, viral particles, and liposomes.
   • Expandable with multiparameter detection modules for simultaneous measurement of binding activity, concentration, and specificity.

Technical Specifications

• Technology: Surface Plasmon Resonance (SPR)
• Kinetic Range: Kon 10³–10⁸ M⁻¹s⁻¹, Koff 10⁻⁶–20 s⁻¹
• Throughput: Up to 384 samples per run (fully automated)
• Data Output: Real-time sensorgrams, kinetic fitting curves, and thermodynamic parameters

Conclusion

The Biacore T200 sets the industry benchmark for biomolecular interaction analysis, combining ultra-high sensitivity, fully automated high-throughput operation, and intelligent data analysis. It is particularly valuable for antibody drug development, small-molecule screening, and biopharmaceutical quality control, providing reliable technical support from basic research to industrial-scale production.