Device built-in

Biacore T100 Biomolecular Interaction Analyzer – English Introduction

Product Overview

The Biacore T100, developed by Cytiva (formerly GE Healthcare Life Sciences), is a high-precision, label-free biomolecular interaction analysis system based on Surface Plasmon Resonance (SPR) technology. It enables accurate measurement of binding kinetics (Kon/Koff), affinity (KD), and thermodynamic parameters for interactions between proteins, nucleic acids, antibodies, small molecules, and other biomolecules. Widely used in drug discovery, antibody screening, signaling pathway research, and quality control, the system provides critical insights into molecular interactions.

Key Features

1. High Sensitivity & Precision

   • Detection limit as low as 1 pg/mm², capable of analyzing weak binding (KD range: 10⁻³–10⁻¹² M) and fast dissociation (Koff up to 10 s⁻¹).
   • Temperature control (4–45°C) supports thermodynamic studies (ΔH/ΔS), ideal for stability assessments.

2. Fully Automated Operation

   • Features auto-sampling, sample recovery, and multi-channel detection, compatible with 96/384-well plates for high-throughput screening.
   • Equipped with Biacore T100 Control Software for real-time data visualization and kinetic fitting (e.g., 1:1 binding, bivalent models).

3. Broad Applications

   • Drug Development: Antibody-antigen affinity optimization, small-molecule inhibitor screening.
   • Basic Research: Protein-protein/nucleic acid interaction mechanisms.
   • GMP Compliance: Meets FDA/EMA regulatory requirements for biologics batch release testing.

4. Flexible Experimental Design

   • Supports multiparameter analysis (e.g., binding activity, concentration determination) with versatile sensor chips (CM5, NTA, SA, etc.) for diverse molecules (e.g., membrane proteins, liposomes).

Technical Specifications

• Technology: Surface Plasmon Resonance (SPR)
• Kinetic Range: Kon 10³–10⁷ M⁻¹s⁻¹, Koff 10⁻⁶–10¹ s⁻¹
• Throughput: Up to 384 samples per run (autosampler)
• Data Output: Real-time sensorgrams, kinetic fitting curves

Conclusion

The Biacore T100 sets the gold standard for biomolecular interaction analysis, combining unmatched sensitivity, automation, and regulatory compliance. It is indispensable for critical quality attribute (CQA) studies in drug development, bridging basic research and industrial-scale biopharmaceutical production.